Animal feed composition and use thereof
A composition and sequence technology, applied in animal feed, animal feed, drug combination, etc., can solve the problems of inability to cut, insolubilize human pathogens, etc.
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Embodiment 1
[0300] Example 1: Determination of muramidase activity
[0301] Muranase activity was measured by measuring the decrease in absorbance / optical density of a solution of suspended Micrococcus lysodeikticus ATTC No. 4698 (Sigma-Aldrich M3770) measured in a microplate reader (Tecan Infinite M200) at 450 nm (decrease) and measured.
[0302] Preparation of Micrococcus lyticus substrate
[0303] Before use, the cells were suspended in deionized water to a concentration of 10 mg cells / mL, and the absorbance / optical density (OD) at 450 nm was measured. The cell suspension was then adjusted so that the cell concentration in the turbidimetric assay (180 μL buffer + 20 μL sample + 20 μL substrate) was equivalent to OD450 = 1.0. The adjusted cell suspension was then stored at ambient temperature until use. Suspended cells were used within 3 hours.
[0304] Preparation of citric acid-phosphate buffer pH 4
[0305] Mix 61.45 mL of 0.1 M citric acid with 38.55 mL of 0.2 M disodium hy...
Embodiment 2
[0309] Embodiment 2: broiler in vivo test 1
[0310] placement and animals
[0311] The trial was carried out from August 15, 2017 to October 3, 2017 in the INTA-EEA Pergamino poultry area of Buenos Aires, Argentina (Poultrz section, INTA-EEA Pergamino, Buenos Aires, Argentina) . One-day-old pullets ("Cobb-500") were supplied by a commercial hatchery (Granja Tres Arroyos, Tres Arroyos 378, Buenos Aires, Argentina).
[0312] Cage
[0313] Poultry cages are housed in traditional barns (with curtains to screen the open sides) in floor pens using wood shavings as litter. Use gas heaters, fans and misters to control barn temperature.
[0314] All poultry were weighed, separated into 1g categories, and distributed to form uniform batches. Poultry with extreme weights are excluded.
[0315] Feed and water were provided ad libitum.
[0316] experimental design
[0317] A randomized complete block design (factor arrangement, 2 levels of enramycin × 2 levels of muramidase) was...
Embodiment 3
[0358] Embodiment 3: broiler in vivo test 2
[0359] Summary of the study
[0360] Feeding trials were conducted using male flocks of 2400 Cobb 400Y chicks for a period of 35 days. The chicks were purchased from a commercial hatchery and were raised on litter in pens throughout the study period. In a fully randomized block design, chicks were assigned to 6 treatment groups and housed within pens (6.5 feet x 4 feet) within the group. Each treatment group consisted of 16 replicate pens with 25 chicks in each pen (n=400 for each treatment and the total number of chicks will be 2400).
[0361] The treatment groups are as follows:
[0362] Table 9: Treatment groups
[0363]
[0364] feed
[0365] All feeds were given in mash form. Implement three-stage feeding: early brooding period (day 1-14 / 500g / poultry), brooding period (day 15-28 / 1500g / poultry) and rearing period (day 29-35 / feed until liquidation) . The rations were prepared from the same batch of raw materials...
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