A marker for detecting quality deterioration of rice stored at high temperature and its application

A marker and rice technology, which is applied in the field of markers to detect the quality deterioration of high-temperature storage rice, can solve the problems that an isolated indicator cannot be used to evaluate its storage quality, the decline of rice eating quality, and the aging and deterioration of rice, etc.

Active Publication Date: 2022-04-22
CHINA AGRI UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

[0003] Although compared with the starch and protein content in rice, lipids are more likely to cause aging and deterioration of rice. During storage, lipids are affected by air and high temperature, which can easily accelerate the rate of rice deterioration, resulting in a decline in the eating quality of rice. Therefore, my country's current rice storage quality judgment rules (GB / T 20569-2006) use the free fatty acid value as a standard to reflect the degree of grain deterioration and storage quality
However, the change of rice storage quality is a dynamic process. Due to the different sensitivities of various indicators during the storage process, various proteins (enzymes) will also have very sensitive changes, and there are different degrees of difference in the impact on the freshness of rice. , can not use an isolated index to evaluate its storage quality, so looking for a new characterization and detection method of rice freshness, to monitor and detect rice quality, to meet consumers' food quality and safety, and to maximize the benefits of full utilization of raw materials for enterprises has important practical significance

Method used

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  • A marker for detecting quality deterioration of rice stored at high temperature and its application
  • A marker for detecting quality deterioration of rice stored at high temperature and its application
  • A marker for detecting quality deterioration of rice stored at high temperature and its application

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Embodiment 1

[0017] The difference in the expression level of rice protein after high temperature storage of embodiment 1

[0018] Rice is generally not only used for local consumption, but a large amount of rice needs to be transported across regions every year. In Northeast China, more than 4 million tons of japonica rice flow out of the province every year, accounting for more than 35% of my country's inter-provincial rice transportation. The transportation methods of rice mainly include sea transportation, railway, rail-water combined transportation, road-water combined transportation and direct road, and 85% of the transportation methods involve container transportation. Generally, the container transportation process takes 2-10 months, and the internal temperature of the container at sea even reaches above 70°C. In order to judge the quality of the rice after storage, the storage conditions of 30°C and 70°C were set, so as to achieve the effect of long-term storage in the shortest t...

Embodiment 2

[0044] (1) Methodology verification: For the protein detected in the sample, after Principal Component Analysis (PCA), calculate the principal component of the protein expression. In the two-dimensional graph, we take the first two principal components PC1, PC2 to represent samples. If the samples are similar, the values ​​of their principal components should also be similar, and they should be close to each other when displayed on the graph, which can also explain the quality of the sample repetition. Depend on figure 1 It can be seen that the protein produced by rice under different storage conditions has good repeatability in the same state, which can significantly distinguish the protein quality.

[0045] (2) Determination of differentially expressed proteins: In the relative quantitative analysis of proteins, if the protein expression abundance ratio is greater than 2.0 or less than 0.5, and the statistical test p<0.01, the protein is defined as a differentially expresse...

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Abstract

The invention discloses a marker for detecting quality deterioration of high-temperature stored rice and an application thereof, belonging to the technical field of grain storage. The present invention obtains the differentially expressed proteins in the rice samples during the storage period by detecting the rice samples under different storage conditions, and further excavates the differential proteins related to the quality deterioration of the stored rice from the perspective of the metabolic pathway through the KEGG pathway analysis, including the abundant proteins in the late stage of embryonic development 19 and glutenin A3, these differential proteins can be used to detect the quality deterioration of high-temperature storage rice.

Description

technical field [0001] The invention belongs to the technical field of grain storage, and in particular relates to a marker for detecting quality deterioration of high-temperature stored rice and an application thereof. Background technique [0002] Rice is one of the important foods for our people. After the rice is ridged, the rice loses the protection of the rice husk, and the endosperm directly contacts with the air. Various changes will occur in the starch, fat and protein in the rice, making the rice lose its original The color, aroma, taste, nutritional content and edible quality of food will decline, and even produce toxic and harmful substances. [0003] Although compared with the starch and protein content in rice, lipids are more likely to cause aging and deterioration of rice. During storage, lipids are affected by air and high temperature, which can easily accelerate the rate of rice deterioration, resulting in a decline in the eating quality of rice. Therefore,...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/36G01N30/72G01N33/573G01N33/68
CPCG01N30/02G01N30/06G01N30/34G01N30/36G01N30/7266G01N33/68G01N33/573G01N2030/8813
Inventor 沈群赵卿宇林佳慧薛勇
Owner CHINA AGRI UNIV
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