Portable microbial induction calcium carbonate precipitation kit and application thereof in repairing of early crack of pavement base course
A kit and calcium carbonate technology, applied in the fields of biochemistry and construction, can solve problems such as poor fluidity, environmental hazards, and easy aging of repair materials.
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[0026] In order to enable those skilled in the art to fully understand the technical solutions and beneficial effects of the present invention, further description will be given below in conjunction with specific embodiments and accompanying drawings.
[0027] The urease-producing bacteria bacterium liquid and various medium formulas involved in the present invention are as follows:
[0028] The urease-producing bacteria liquid is mixed by urease-producing bacteria, peptone, yeast extract, sodium chloride and sterile water, wherein the content of peptone is 8-10g / L, and the content of yeast extract is 3-5g / L L, the content of sodium chloride is 8-10g / L, and the bacterial liquid OD measured by ultraviolet spectrophotometer 600 = 1.3-1.8.
[0029] LB solid medium (by weight): 8-10 parts of peptone, 3-5 parts of yeast extract, 8-10 parts of sodium chloride, 15-20 parts of agar powder, 1000 parts of distilled water, pH=7.2-7.4 .
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