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Application of marker in preparation of diagnostic kit or therapeutic drug for rheumatoid arthritis

A technology of diagnostic kits and therapeutic drugs, applied in the field of disease diagnosis

Active Publication Date: 2021-05-11
THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, only some patients can achieve disease remission, and it is necessary to continue to study its pathogenesis and find new therapeutic targets

Method used

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  • Application of marker in preparation of diagnostic kit or therapeutic drug for rheumatoid arthritis
  • Application of marker in preparation of diagnostic kit or therapeutic drug for rheumatoid arthritis
  • Application of marker in preparation of diagnostic kit or therapeutic drug for rheumatoid arthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] 1.1 Exosome extraction and identification

[0059] (1) Culture of human RA FLS and OA FLS:

[0060] Case source Six RA patients and six knee osteoarthritis (OA) patients underwent knee arthroplasty in the Department of Orthopedics, Second Xiangya Hospital, Central South University. The diagnosis of RA conforms to the classification criteria of RA revised by the American College of Rheumatology (ACR) in 1987. The diagnosis of OA was in line with the 1986 ACR revised knee OA classification criteria. This study was approved by the Ethics Committee of the Second Xiangya Hospital of Central South University.

[0061] Aseptically obtain the synovial tissue, cut it into pieces and add collagenase type Ⅲ (4mg.ml -1 ) digested, filtered and centrifuged, cultured with 10% FBS culture medium DMEM, after the cells grew 80%-90%, digested with 0.25% trypsin and passaged 3-8 generation cells for experiment. The expression of CD3, CD14 and CD90 antigens on the cell surface was dete...

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Abstract

The invention relates to the field of disease diagnosis technology and preparation of therapeutic drugs, in particular to application of a marker in preparation of a diagnostic kit or therapeutic drug for rheumatoid arthritis. In the application, the marker is a synovial cell supernate exosome lncRNA SNHG5 or a truncated sequence thereof; and the sequence of the lncRNA SNHG5 is as shown in SEQ ID NO: 1. The research finds that the expression of the synovial cell exosome lncRNA SNHG5 in rheumatoid arthritis patients is obviously increased compared with that in osteoarthritis patients, and by means of the exosome lncRNA SNHG5, a therapeutic target and an important basis are provided for not only early-stage rapid detection of the rheumatoid arthritis, but also clinical application such as gene therapy and drug therapy.

Description

technical field [0001] The invention relates to the technical field of disease diagnosis, in particular to the application of a marker in the preparation of a rheumatoid arthritis diagnostic kit or therapeutic drug. Background technique [0002] Rheumatoid Arthritis (RA) is a chronic autoimmune disease with synovium as the main target tissue. The morbidity rate of the course of disease 5-10 years is as high as 60%. The high disability of RA and its social and economic burden make RA a public health problem of global concern. With the in-depth research on the pathological mechanism of RA, the biological therapy targeting cytokines, intracellular signaling pathways and inflammatory cells has achieved great development, which has improved the curative effect of RA. However, only some patients can achieve disease remission, and it is necessary to continue to study its pathogenesis and find new therapeutic targets. [0003] Exosomes are nano-membrane capsules (30-100nm) secret...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/6851A61K45/00A61P19/02A61P29/00
CPCC12Q1/6883C12Q1/6851A61K45/00A61P19/02A61P29/00C12Q2600/158C12Q2600/178C12Q2600/136C12Q2531/113C12Q2521/107C12Q2563/107C12Q2545/101
Inventor 葛燕田静阳璧玲
Owner THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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