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Plant mitochondrial genome structure annotation method

A mitochondrial genome and annotation technology, applied in genomics, proteomics, bioinformatics, etc.

Pending Publication Date: 2021-05-11
南京集思慧远生物科技有限公司
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Problems solved by technology

[0005] The present invention provides a method for annotating plant mitochondrial genome structure, aiming to solve the problems mentioned in the above-mentioned background technology

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  • Plant mitochondrial genome structure annotation method
  • Plant mitochondrial genome structure annotation method
  • Plant mitochondrial genome structure annotation method

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Embodiment 1

[0056] A method for annotating the mitochondrial genome structure of bell child, comprising the following steps:

[0057] S1. Annotate the coding sequence (CDS), open reading frame (ORF), transfer RNA (tRNA) and ribosomal RNA (rRNA) using Bell's mitochondrial genome sequence and corresponding software respectively;

[0058] S2. Homologous annotation of bell mitochondrial genome coding sequence (CDS): use blastn (v2.10.1) software and the constructed plant mitochondrial genome coding sequence (CDS) library to compare the mitochondrial genome sequences, and select the best alignment A sequence with a similarity of more than 90% is used as the annotation result, and the position of the promoter and terminator is corrected for the coding sequence (CDS) on the annotation, and the position on the genome is determined. Using the species Nicotiana tabacum (BA000042), pepper (Capsicum annuum, KJ865410), Tianxianzi (Hyoscyamus niger, NC_026515), tomato (Solanum lycopersicum, NC_035963),...

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Abstract

The invention is applicable to the technical field of plant mitochondria, and provides a plant mitochondrial genome structure annotation method which comprises the following steps: S1, respectively carrying outannotation ofa coding sequence (CDS), an open reading frame (ORF), transport RNA (tRNA) and ribosome RNA (rRNA) by using a plant mitochondrial genome sequence and corresponding software; s2, carrying out coding sequence (CDS) homologous annotation; s3, carrying out open reading frame (ORF) annotation; s4, carrying out transfer RNA (tRNA) annotation; s5, carrying out ribosome RNA (rRNA) annotation; s6, fusing annotation results of S2, S3, S4 and S5 to obtain structure annotation and function annotation results of the plant mitochondrial genome, and generating structure annotation and function annotation files of the plant mitochondrial genome in genbank and tbl formats. According to the annotation method, detailed annotation information can be quickly obtained from the complete plant mitochondrial genome, and the annotation method is suitable for most plant mitochondrial genomes, so that research and application in related fields based on mitochondrial data are promoted.

Description

technical field [0001] The invention belongs to the technical field of plant mitochondria, in particular to a method for annotation of plant mitochondrial genome structure. Background technique [0002] Plant mitochondria are an important place for plants to carry out respiratory metabolism and life activities, and their genome is relatively independent of the nuclear genome, showing semi-autonomous inheritance. All plant mitochondrial genomes contain a set of essential genes related to mitochondria maintaining their own growth and development and performing their special functions. Genes generally required for the maintenance of the major mitochondrial functions electron transport and oxidative phosphorylation are well conserved across species. Plant mitochondrial genes not only have multiple copies, but also introns, and there are trans-splicing in introns. The absence of introns in mitochondrial genes of other species such as animals is one of the characteristics of mit...

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Application Information

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IPC IPC(8): G16B20/20G16B30/10
CPCG16B20/20G16B30/10
Inventor 高海东周向阳徐雷
Owner 南京集思慧远生物科技有限公司
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