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Method for producing drimanyl acetate compounds

A technology for replenishing alkyl alcohols and alkyl esters, applied in biochemical equipment and methods, acyltransferases, enzymes, etc., can solve the problems of activity dependence, lipase cannot catalyze transesterification reactions, etc.

Pending Publication Date: 2021-05-11
FIRMENICH SA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, under physiological conditions, lipases, to the best of our knowledge, cannot catalyze the desired transesterification reaction
Although ester formation is possible by lipase, the activity strongly depends on the amount of water present

Method used

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  • Method for producing drimanyl acetate compounds
  • Method for producing drimanyl acetate compounds
  • Method for producing drimanyl acetate compounds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0098] 1. produces at least one, in particular one, two or three, in particular ester compound of biocatalytic processes one or two alkyl acid supplement,

[0099] Includes the following steps:

[0100] (1) in the presence of an acetyl donor, at least one, in particular a stereomerically pure form or stereo isomer mixtures in the form of a supplement of at least one alkyl alcohol, in particular in contact with a polypeptide the polypeptide is capable of having a particular from the acetyl donor acetyl transferred to the at least one, in particular one alkyl alcohol supplement acetyltransferase activity, to thereby obtain as a major product alkyl esters of acetic acid supplement, in particular a complement-acetic acid as the major product body alkyl; and

[0101] (2) optionally, separating the reaction product from step (1) of the at least one, in particular one supplement acid alkyl ester compound.

[0102] If more than one form of alkyl esters of acetic acid supplement, the mixtu...

Embodiment 1

[0409] The acetyl transferase candidate was selected to convert the bifurcane iliac to a bachelipide.

[0410] Acetyl transferase constitutes a relatively varied enzyme, known for more than 8,000 species (PFAM database: PF02458 transferase family). Although the libraries of the molecules that are subjected to a substrate by acetyl transferase are large, there is no compassionate of semiphypel alcohol as a substrate. In order to identify acetyl transferase which is capable of acetylation-backed bifurcane type chippel alcohol, fifty-four kinds of acetyl transferase from thousands of known destined derived from plants, fungi and bacteria are selected based on the following principles. 1):

[0411] The identified members of the two-evilted branches 3 and 5--evilted branches 3 and 5-, which were known from the plant acetyl transferase BaHD family were carried out using acetyl coenzyme A as an acyl donor donor donor donor donor donor donor donor donor donor donor donor donor Curr Opin ...

Embodiment 2

[0420] In vivo acetate Generation of leafolar ester.

[0421] Each enzymatic gallery was screened to carry out the biological conversion of the in vitro tobacle alcohol to acetic acid. In order to screen, acetyl transferase candidates with acetyl transferase candidate and a coded gene of Dichomitus Squalens, a coding gene of the coding gene of Dichomitus Squalens, NCBI login XP_007369631.1 Common expressed, in this strain, an endogenous method of endogenous method (FPP) was improved.

[0422] In order to increase the level of endogenous FPP library in breweled yeast cells, an additional copy of all yeast endogenous genes of a methylene hydroxylic acid, from ERG10 encoded acetyl covidase A C-acetyl transferase, ERG20 encoded FPP synthase Similar to Paddon et al., Nature, 2013, 496: 528-532, consolidated to Saccharomycemic strain CEN.PK2-1C (Euroscarf, Frankfurt, Germany) under the control of galactose inducible promoters The genome. In short, three boxes are integrated in LEU2, T...

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Abstract

The present invention provides novel methods for the acetyl transferase-catalyzed production of drimanyl- acetate compounds by the acetylation of the respective drimanyl alcohol sources performed in vitro or in vivo. The present invention also relates to the identification of enzymes having corresponding acetyl transferase activity from different microbial and plant sources. The present invention also relates to the provision of enzyme mutants derived from said newly identified enzymes. A further aspect of the present invention relates to the provision of corresponding coding sequences of such enzymes and mutants, recombinant vectors, and recombinant host cells suitable for the production of such acetyl transferases and mutants and for performing the novel production methods of drimanyl acetate compounds. Another aspect of the invention relates to the use of such drimanyl acetates, as obtained according to the present invention, as intermediates for the production of odorant, flavor or fragrance or insect / pest control ingredients.

Description

Technical field [0001] The present invention provides a new method for performing acid acetyltransferase enzymatic supplement the corresponding alkyl (drimanyl) acetylated alcohol derived in vitro or in vivo to produce a supplement alkyl ester compound. The present invention further relates to a corresponding enzyme having acetyl transferase activity from different microbial identification and plant sources. The present invention is also directed to providing an enzyme derived from the newly identified mutants. Another aspect of the invention relates to corresponding coding sequences and an enzyme to provide such a mutant, recombinant vectors and recombinant host cells, which are adapted to produce such acetyltransferase and a mutant acid supplement, and for the alkyl ester compound new generation method. Another aspect of the invention relates to a supplement according to the present invention, an alkyl ester of acetic acid obtained in this use as an intermediate for producing od...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/62C12N9/10
CPCC12N9/1025C12P7/62C12Y203/00C12P7/02
Inventor D·索利斯艾斯卡兰特C·格纳L·戴维特王祺
Owner FIRMENICH SA
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