A strain of Lactobacillus plantarum and its application in preparing medicine or food for alleviating diseases caused by hyperlipidemia
A technology of Lactobacillus plantarum and hyperlipidemia, applied in the application field of medicine, can solve the problems of large side effects and high cost, and achieve the effects of improving liver function, increasing the content and reducing the incidence rate
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Embodiment 1
[0026] Example 1 Isolation and Identification of Lactobacillus plantarum HH19058
[0027] 1. Separation, purification and morphological identification
[0028] Pickle juice: In September 2018, cabbage pickles were collected from farmers in Qingdao, Shandong, and the pickle juice obtained by homogenizing the leaves and juice was diluted 10 times in a gradient.
[0029] Use MRS solid medium, LBS solid medium and NPNL solid medium to isolate the lactic acid bacteria in the above pickle juice, culture the isolated lactic acid bacteria in an anaerobic workstation at 37°C for 48 hours, pick typical colonies on the plate and streak to separate them to obtain pure Colonies were observed morphologically by Gram staining, and cultured anaerobically in MRS liquid medium at 37°C for 24h, and refrigerated at 4°C for later use.
[0030] The result is as figure 1 with figure 2 As shown, the purified strain HH19058 formed a single colony in the MRS medium, and the colonies were almost uni...
Embodiment 2
[0056] Example 2 The Research of Lowering Cholesterol and Triglycerides by Lactobacillus plantarum HH19058
[0057] Inoculate Lactobacillus plantarum HH19058 into cholesterol medium (MRS-TC) at 37°C for 24 hours at 3% inoculum, take 0.2 mL of the culture solution, add 4.8 mL of absolute ethanol and shake and mix for 1 min, then shake and mix after standing for 5 min. Evenly, centrifuge at 4000r / min for 10min, take 2.0mL supernatant for the determination of cholesterol, and use uninoculated cholesterol medium as a control.
[0058] Lactobacillus plantarum HH19058 was inoculated into triglyceride medium (MRS-TG) at 3% inoculum size and cultured at 37°C for 24 hours, then centrifuged at 4000r / min for 10min, and 2.0mL of supernatant was used for the determination of triglyceride. Inoculated triglyceride medium served as a control.
[0059] Cholesterol medium (MRS-TC) preparation: 0.1g of cholesterol, 1.0mL of Tween-20, 0.1g of sucrose ester, 5mL of glacial acetic acid, sonicated ...
Embodiment 3
[0062] Example 3 Effect of Lactobacillus plantarum HH19058 on mice with hyperlipidemia
[0063] Select 108 qualified C57 adult mice, 13 weeks old, half male and half male, the difference in body weight of the same sex should be within 20% of the average body weight, and after adaptive feeding with basal feed for 1 week, according to the average body weight of each group Significant differences were classified into 3 groups (Table 2). Group 1 was fed with normal basal diet, and groups 2 and 3 were fed with high-fat diet, and a mouse model of hyperlipidemia was established by feeding high-fat diet for 4 weeks.
[0064] Table 2 Animal Modeling Grouping
[0065]
[0066] After the model was established, the mice were gavaged at a fixed time every morning according to the treatment method in Table 2 for 4 consecutive weeks, at a rate of 0.1 mL / 10g body weight. During the experiment period, the body weight was weighed every 7 days, and the gavage volume was adjusted.
[0067] ...
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