Application of blood-transforming elixir in mite and insect killing, biological prevention and control medicament, and preparation method of biological prevention and control medicament
A kind of technology of Huaxuedan, which is applied in the direction of botany equipment and methods, insecticides, acaricides, etc., can solve the problems that there are no reports of Huaxuedan killing pests, and achieve the effect of preventing and controlling pests and mites
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0086] The embodiment of this group provides a preparation method of biological control agent. All the examples in this group have the following common features: the preparation method includes: obtaining Huaxuedan by distillation and extraction.
[0087] In a specific embodiment, said refers to distillation by boiling for 8-12 hours to obtain an oil-water mixture;
[0088] Preferably, the extraction refers to extracting the oil-water mixture obtained by distillation with n-hexane;
[0089] Preferably, the temperature of the water bath for the extraction is 35-45°C, preferably 40°C;
[0090] Preferably, the Huaxuedan is chopped and soaked before boiling, so that essential oil can be obtained as much as possible;
[0091] Preferably, boil for 10 hours
[0092] Preferably, the extracted mixture is dried and evaporated to remove n-hexane;
[0093] Preferably, the drying refers to drying with anhydrous sodium sulfate;
[0094] Preferably, the evaporation refers to evaporation...
experiment example 1
[0095] Experimental example 1, the preparation method of Huaxuedan essential oil
[0096] In the present invention, a method for extracting Huaxuedan essential oil is provided, but it is only used to realize the purpose of the present invention and is not limited to the extraction of this method.
[0097] After chopping 8 kg of Huaxuedan plant (dried plant purchased from Yunyao.com, not processed), distilled by boiling for 8-12 hours, the obtained oil-water mixture was extracted with n-hexane, and the extracted product was dehydrated with anhydrous sodium sulfate , Use a rotary evaporator to evaporate and concentrate under reduced pressure in a water bath at 40-45°C, recover n-hexane, and the resulting residual oily liquid is the volatile oil of Huaxuedan.
experiment example 2
[0098] Experimental example 2, GC-MS detection of Huaxuedan essential oil
[0099] The Huaxuedan essential oil prepared in Example 1 was sent to Beijing Normal University Temperament Testing Center for testing.
[0100] Agilent6890N gas chromatograph and Agilent5973N mass spectrometer were used.
[0101] HP-5MS 30mm×0.25mm×0.25μm quartz capillary column, the initial column temperature is 60°C for 1 minute, the temperature is programmed to 180°C at a rate of 12°C / min and maintained for 0.5 minutes, and then the temperature is programmed to 280°C at a rate of 25°C / min ℃ for 18 minutes, vaporizer temperature 280 ℃, carrier gas helium, column flow 1.0mL / min, split ratio 1:10.
[0102] The mass spectrometer ionization mode is EI, the electron bombardment energy is 70eV, the scanning mass range is 20-550m / z, and the scanning time is 2 seconds.
[0103] 1 μL (500 times n-hexane dilution) of Huaxuedan plant essential oil was sampled, analyzed and identified by gas chromatography-mas...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More