Application of Globba racemosa in mite and insect killing, biopesticide and preparation method thereof
A kind of biological pesticide and related technology, which is applied in the fields of botanical equipment and methods, insecticides, acaricides, etc., can solve the problems such as the killing of pests by dancing flower ginger, and achieve the effect of preventing and controlling pests and mites.
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preparation example Construction
[0083] The 4th group of embodiment, the preparation method of biopesticide of the present invention
[0084] The embodiment of this group provides a kind of preparation method of biopesticide. All the examples in this group have the following common features: the preparation method includes: boiling and extracting Wuhua ginger.
[0085] In a specific embodiment, the oil-water mixture is obtained by boiling for 8-12 hours;
[0086] Preferably, the extraction refers to extracting the oil-water mixture obtained by distillation with n-hexane;
[0087] Preferably, the temperature of the water bath for the extraction is 35-40°C, preferably 40°C;
[0088] Preferably, the dancing ginger is sliced before the boiling;
[0089] Preferably, the underground part of the dancing ginger is sliced and boiled for 8-12 hours.
[0090] Preferably, the extracted mixture is dried and evaporated to remove n-hexane;
[0091] Preferably, the drying refers to drying with anhydrous sodium sulfa...
experiment example 1
[0093] Experimental example 1, the preparation method of dancing ginger essential oil
[0094] In the present invention, a kind of extracting method of ginger essential oil is provided, but only for the essential oil of ginger dance to realize the purpose of the present invention, not limited to the extraction of this method.
[0095] Slice the underground part of 5000g of fresh Dancing Ginger, extract the oil-water mixture obtained by boiling with n-hexane, dehydrate the extracted product with anhydrous sodium sulfate, and decompress in a water bath at 40-45°C with a rotary evaporator Evaporation and concentration, recovery of n-hexane, the resulting residual oily liquid is the volatile oil of Wuhuajiang.
experiment example 2
[0096] Experimental example 2, GC-MS detection of dancing ginger essential oil
[0097] The Dancing Ginger essential oil prepared in Example 1 was sent to Beijing Normal University Temperament Testing Center for testing.
[0098] Agilent6890N gas chromatograph and Agilent5973N mass spectrometer were used.
[0099] HP-5MS 30mm × 0.25mm × 0.25μm quartz capillary column, the initial column temperature is 60°C for 1 minute, the temperature is programmed to 180°C at a rate of 12°C / min and maintained for 0.5 minutes, and then the temperature is programmed to 280°C at a rate of 25°C / min ℃ for 18 minutes, vaporizer temperature 280 ℃, carrier gas helium, column flow 1.0mL / min, split ratio 1:10.
[0100] The mass spectrometer ionization mode is EI, the electron bombardment energy is 70eV, the scanning mass range is 20-550m / z, and the scanning time is 2 seconds.
[0101] Take 1 μL (500-fold n-hexane dilution) of the plant essential oil of Gingeria japonicus as a sample, analyze and ide...
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