The use of arrow shaft wind in killing mites and insecticides and its biological control agent and preparation method
An arrow shaft wind and chemical technology, which is applied in the fields of botanical equipment and methods, insecticides, acaricides, etc., can solve the problems such as reports that the arrow shaft wind kills pests has not yet appeared.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0084] The embodiment of this group provides a preparation method of biological control agent. All the examples in this group have the following common features: the preparation method includes: distilling and extracting arrow shaft wind.
[0085] In a specific embodiment, the oil-water mixture is obtained by boiling in water for 8-12 hours;
[0086] Preferably, the extraction refers to extracting the oil-water mixture obtained by distillation with n-hexane;
[0087] Preferably, the temperature of the water bath for the extraction is 35-45°C, preferably 40°C;
[0088] Preferably, before the distillation, the arrow shaft is wind-cut and soaked to obtain the essential oil;
[0089] Preferably, the extracted mixture is dried and evaporated to remove n-hexane;
[0090] Preferably, the drying refers to drying with anhydrous sodium sulfate;
[0091] Preferably, the evaporation refers to evaporation at 40-45° C. using a rotary evaporator.
experiment example 1
[0092] Experimental example 1, the preparation method of arrow shaft wind essential oil
[0093] In the present invention, a method for extracting the essential oil of arrow shaft wind is provided, but it is only used to realize the purpose of the present invention, and is not limited to the extraction of this method.
[0094] Cut 5000g arrow shafts into small pieces and soak them thoroughly, then boil them for 8-12 hours, extract the obtained oil-water mixture with n-hexane, dehydrate the extracted product with anhydrous sodium sulfate, and use a rotary evaporator at 40-45°C Evaporate and concentrate under reduced pressure in a water bath pot, recover n-hexane, and the obtained residual oily liquid is Arrowwind volatile oil.
experiment example 2
[0095] Experimental example 2, GC-MS detection of arrow shaft wind essential oil
[0096] The Arrowwind essential oil prepared in Experimental Example 1 was sent to the Temperament Testing Center of Beijing Normal University for testing.
[0097] Agilent6890N gas chromatograph and Agilent5973N mass spectrometer were used.
[0098] HP-5MS 30mm×0.25mm×0.25μm quartz capillary column, the initial column temperature is 60°C for 1 minute, the temperature is programmed to 180°C at a rate of 12°C / min and maintained for 0.5 minutes, and then the temperature is programmed to 280°C at a rate of 25°C / min ℃ for 18 minutes, vaporizer temperature 280 ℃, carrier gas helium, column flow 1.0mL / min, split ratio 1:10.
[0099] The mass spectrometer ionization mode is EI, the electron bombardment energy is 70eV, the scanning mass range is 20-550m / z, and the scanning time is 2 seconds.
[0100] Take 1 μL (100 times acetone dilution) of Arrowwind plant essential oil as a sample, analyze and identi...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More