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Hydrogen peroxide detection method

A hydrogen peroxide and concentration technology, which is applied in material analysis by observing the influence of chemical indicators, analysis by chemical reaction of materials, measuring devices, etc., can solve problems such as lack of detection of hydrogen peroxide, and achieve high efficiency. The effect of sensitive detection

Pending Publication Date: 2021-05-28
NINGDE NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is still a lack of efficient and sensitive methods for detecting hydrogen peroxide in the prior art.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Weigh 1.4644mg of L-valine into a 50mL clean beaker, add 34mL of secondary water, stir evenly, and then transfer it into a 50mL polytetrafluoroethylene reactor. React in an oven at 200°C for 12 hours and cool to room temperature. Purify, and the purified carbon quantum dots (CODs) are stored in a refrigerator for future use.

[0050] The fluorescent properties of the synthesized CODs were observed using a F-7000 fluorescence photometer from Japan HITACHI Company. Use a xenon lamp as the excitation light source, set the PMT voltage to 700V, the slit of the excitation light is 5nm, the slit of the emission light is 5nm, and the scanning speed is 1200nm / min. In the fluorescence cuvette, measure the fluorescence spectrum. Use UV-2600 model UV-absorption spectrophotometer to observe the UV absorption peak of synthesized CODs. The wavelength range set by the instrument is 200-800nm. Under UV light irradiation, the valine-CQDs emit bright blue light. Depend on figure 1 I...

Embodiment 2

[0053] TMB, which is relatively stable in nature, was used as the enzyme-catalyzed substrate, and H 2 o 2 as an oxidant to explore the peroxidase-like activity of the synthesized CQDs. When no CQDs were added, TMB-H 2 o 2 The mixed solution was colorless and transparent, but after adding CQDs, the solution turned blue and the color deepened with time, which confirmed that CQDs had peroxidase-like catalytic activity; in addition, when there was no H in the system 2 o 2 , TMB does not show reaction when only CQDs are present ( image 3 ), which indicated that CQDs were not oxidants and could not cause TMB to undergo a display reaction. In addition, in order to further study the catalytic activity of CQD peroxidase-like, the change of absorbance of TMB oxidation products under different reaction time conditions was investigated ( Figure 4 ). Figure 4 Shown is the full spectrum of UV absorption at 3-minute intervals. by TMB-H 2 o 2 - UV-vis absorption spectrum of CQDs ...

Embodiment 3

[0055] The peroxidase-like enzymes at different temperatures (32-37°C), different pHs (3.6-5.8), different TMB concentrations (0.05-0.85mmol / L), and different carbon dot concentrations (0.17-0.657mmol / L) were investigated Active influence.

[0056] The temperature of the solution has a great influence on the catalytic activity of the enzyme. In order to investigate the change of the temperature on the system, this experiment was carried out in a low-temperature constant temperature water bath, and the temperature was tested from 32°C to 67°C. The test results are shown in 5. Show. The results showed that in the range of 32-37°C, as the temperature increased, the catalytic activity also increased; while when the temperature was higher than 57°C, the catalytic activity gradually decreased, because the decomposition rate of hydrogen peroxide accelerated as the temperature increased.

[0057] pH affects the activity of CQDs catalytic enzymes. Therefore, the change value of TMB ab...

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Abstract

The invention provides a hydrogen peroxide detection method, which comprises: S1, mixing valine and secondary water, and carrying out hydrothermal reaction to prepare carbon quantum dots; S2, preparing a detection solution containing carbon quantum dots and TMB; S3, taking a certain amount of the detection solution, adding H2O2 with different concentrations into the detection solution, carrying out reaction for a period of time, then measuring the absorbance, and making a standard curve of the H2O2 concentration and the absorbance; and S4, taking a to-be-detected sample containing H2O2, adding the to-be-detected sample containing H2O2 into a same amount of the detection solution, carrying out reaction for the same time, measuring the absorbance, and calculating the content of H2O2 in the to-be-detected sample according to the standard curve. The method can effectively detect the concentration of H2O2.

Description

technical field [0001] The invention relates to a method for detecting hydrogen peroxide, belonging to the technical field of hydrogen peroxide detection. Background technique [0002] Hydrogen peroxide (H 2 o 2 ) is a colorless liquid, is a strong oxidizing agent, can inhibit the growth of bacteria, and can be widely used in industrial and medical fields as a stabilizer or preservative. However, in biochemical reactions, it can produce a large number of hydroxyl radicals, participate in electron transfer and hydroxylation reactions, and cause cell necrosis or mutation, which is very harmful to the human body. Therefore, the use in the food field should be strictly controlled. Control to avoid excess hydrogen peroxide residue. Therefore, it is of great significance to establish an efficient and sensitive method for the detection of hydrogen peroxide in food. However, there is still a lack of efficient and sensitive methods for detecting hydrogen peroxide in the prior art...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/78
CPCG01N21/31G01N21/78G01N2021/786
Inventor 李萍
Owner NINGDE NORMAL UNIV
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