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Jade in-vitro efficacy evaluation method

A technology for evaluating the efficacy of jade and jade, applied in the field of medicine, can solve problems such as difficulty in effectively evaluating the efficacy of jade in vitro, and achieve good application prospects and the effect of inhibiting or promoting cell proliferation.

Pending Publication Date: 2021-06-01
北京赛赋医药研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] A main purpose of the present invention is to overcome at least one defect of the above-mentioned prior art, and provide a method for evaluating the efficacy of jade in vitro, so as to solve the problem that the existing methods are difficult to effectively evaluate the efficacy of jade in vitro

Method used

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  • Jade in-vitro efficacy evaluation method
  • Jade in-vitro efficacy evaluation method
  • Jade in-vitro efficacy evaluation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] 1) Cell culture (including recovery and passage)

[0051] Cell recovery process: Take out the human neuroblastoma SH-SY5Y cell cryopreservation tube from the liquid nitrogen tank, quickly put it into a 37°C water bath, shake gently until it is completely melted, then take it out, draw the cell suspension into the centrifuge tube, Add 2mL of culture medium, centrifuge at 1000rpm / min for 5min, remove the supernatant, add 2mL of MEM complete medium, pipette evenly, transfer to a culture bottle, make up the medium, and place at 37°C, 5% CO 2 Cultivate in an incubator and observe the next day to see if the cells need to be cultured, or whether the cells need to be replaced or passaged.

[0052] Cell subculture process: After the cells are recovered, when the adherent cells cover about 80% to 90% of the bottom of the bottle, the cells need to be subcultured, suck out the culture medium in the bottle, wash twice with sterile PBS, and remove the residual medium , add an approp...

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Abstract

The invention provides a jade in-vitro efficacy evaluation method which comprises the following steps: providing a transwell chamber which comprises an upper chamber and a lower chamber; adding cells and a culture medium into the lower chamber, adding a jade-containing solution into the upper chamber, and standing for drug incubation; and taking out the upper chamber, detecting the cell viability in the lower chamber, and calculating the cell viability. According to the method, cell and medicine co-incubation is carried out by utilizing a transwell chamber, effective components in the jade extract can act on cells in the lower chamber to inhibit or promote cell proliferation, and other impurities do not influence judgment of test results. The method is of great significance to in-vitro pharmacodynamic study of jade and has a good application prospect.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a method for evaluating drug efficacy in vitro of jade. Background technique [0002] For a long time, in pharmacological research, cell-based in vitro drug activity detection is indispensable, especially for drug screening, mechanism analysis is a necessary technology. [0003] When it comes to the detection of the effect of drugs on cells, generally after the cells are inoculated into the cell culture plate, the drugs are directly in contact with the cells, and the cell activity is detected by using MTT and CCK-8. Pharmaceutical activity testing in water. However, the composition of the jade to be detected is complex, and it is difficult to extract and obtain the extract. The general detection method causes the particles to directly contact the cells. On the one hand, it affects the state of the cells. On the other hand, the particles are adsorbed on the bottom of the plate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02C12M3/06C12M1/34
CPCG01N33/5008C12M29/04C12M41/46C12N2503/02G01N2500/10
Inventor 杜杰高香云王征林春轶
Owner 北京赛赋医药研究院有限公司