Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preventing and treating culex pipiens pallens by utilizing beauveria bassiana

A technology for Beauveria bassiana and Culex pipiens pallens is applied in the directions of using spores, microorganism-based methods, pest control, etc., and can solve the problems of increasing the range of activities, increasing the difficulty of mosquito control, and achieving strong resistance. ability, enhance the mosquito-inducing function, enhance the effect of attracting range

Active Publication Date: 2021-06-04
山东省寄生虫病防治研究所
View PDF6 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Adult mosquitoes have a strong ability to travel, and their range of activities has greatly increased, which increases the difficulty of mosquito control

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preventing and treating culex pipiens pallens by utilizing beauveria bassiana
  • Method for preventing and treating culex pipiens pallens by utilizing beauveria bassiana
  • Method for preventing and treating culex pipiens pallens by utilizing beauveria bassiana

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A kind of method utilizing Beauveria bassiana to control Culex palustris, it comprises the following steps:

[0027] 1) Crush 10g of kelp into 40 mesh, add 100mL of water, alternately freeze and thaw twice, extract in water at 90°C for 6 hours, and vacuum filter to obtain kelp filtrate; dry the leftovers of fish and shrimp, and crush them into 80 mesh to obtain For fish and shrimp powder, put 50mL of kelp filtrate, 1g of fish and shrimp powder, 1g of algae extract, 1g of yeast extract powder and 2g of activated carbon powder into 200mL of distilled water, mix well, and inoculate with 3-5% Beauveria bassiana and cultured on a shaker at 25°C for 5-7 days to obtain a culture of Beauveria bassiana;

[0028]2) Centrifuge the culture of Beauveria bassiana and collect the sediment. Mix 30g of the Beauveria bassiana sediment with 2g of brown sugar powder, 1g of algae extract and 0.5g of yeast extract powder, and carry out at 30°C. Dry to obtain a Beauveria bassiana mixture; di...

Embodiment 2

[0033] A kind of method utilizing Beauveria bassiana to control Culex palustris, it comprises the following steps:

[0034] 1) Crush 10g of kelp into 50 mesh, add 100mL of water, alternately freeze and thaw twice, extract in water at 90°C for 6 hours, and vacuum filter to obtain kelp filtrate; dry the leftovers of fish and shrimp, and crush them into 100 mesh to obtain For fish and shrimp powder, put 50mL of kelp filtrate, 1g of fish and shrimp powder, 1g of algae extract, 1g of yeast extract powder and 2g of activated carbon powder into 200mL of distilled water, mix well, and inoculate with 3-5% Beauveria bassiana and cultured on a shaking table at 25° C. for 7 days to obtain a culture of Beauveria bassiana; wherein, the preparation method of the algae extract is to prepare Chlorella, Anabaena, Kujin Crescent, Scenedesmus fusarium , Spleenia chrysalis, S. mulberry, Scenedesmus bulge and Scenedesmus smithii were dissolved in 75% ethanol solution, alternately frozen and thawed ...

Embodiment 3

[0040] A kind of method utilizing Beauveria bassiana to control Culex palustris, it comprises the following steps:

[0041] 1) Crush 10g of kelp into 40 mesh, add 100mL of water, freeze and thaw twice alternately, extract in water at 90°C for 6 hours, and vacuum filter to obtain the kelp filtrate; dry the leftovers of fish and shrimp, and crush them into 100 mesh to obtain Fish and shrimp powder, put 50mL of kelp filtrate, 1g of fish and shrimp powder, 1g of algae extract, 1g of yeast extract powder and 2g of activated carbon powder in 200mL of distilled water, mix evenly, inoculate with 3% Beauveria bassiana and place in Shaking bed culture was carried out at 25°C for 5 days to obtain a culture of Beauveria bassiana; wherein, the preparation method of the algae extract was to prepare Chlorella, Anabaena, Kujin Crescent, Scenedesmus fusarium, Golden Shield Dinosaurus, Esterococcus mulberry, Scenedesmus bulge and Scenedesmus smithii were dissolved in 75% ethanol solution, froze...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Densityaaaaaaaaaa
Densityaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for preventing and treating culex pipiens pallens by utilizing beauveria bassiana. The method comprises the following steps: 1) culturing the beauveria bassiana to obtain a beauveria bassiana culture; 2) centrifuging the beauveria bassiana culture, collecting a precipitate, uniformly mixing the beauveria bassiana precipitate with 2g of brown powdered sugar, 1g of an algae extract and 0.5 g of yeast extract powder, and performing drying at 30-40 DEG C to obtain a beauveria bassiana mixture; dissolving 100g of polylactic acid in 500mL of acetone to obtain a polylactic acid solution; uniformly mixing the beauveria bassiana mixture, polylactic acid and urea with different contents to prepare film spraying liquid containing urea with different contents; (3) preparing pellet cores by using the expandable polystyrene beads; spraying films on the pellet cores for multiple times to obtain mosquito prevention and control particles; 4) scattering the mosquito prevention and control particles into a water body needing mosquito prevention and control to realize mosquito prevention and control. The mosquito trap has the effects of trapping mosquito larvae and killing mosquitoes.

Description

technical field [0001] The invention belongs to the field of mosquito control. More specifically, the present invention relates to a method of using Beauveria bassiana to control Culex palustris. Background technique [0002] Mosquitoes belong to Diptera, Mosquitoidae, also known as mosquitoes. Mosquito bites not only cause reactions such as redness, swelling, and itching, but may also cause the spread of diseases such as malaria, dengue fever, filariasis, Zika virus disease, and Japanese encephalitis, which seriously endanger human health. The life cycle of mosquitoes can be divided into four stages, namely egg, larva, pupa and adult. Mosquitoes live in water in the first three periods, while adults live on land, and female mosquitoes need to lay eggs in stagnant water, and mosquito eggs must be in water to hatch. Adult mosquitoes have a strong ability to travel, and their range of activities has increased significantly, increasing the difficulty of mosquito control. Th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01N63/30A01N37/36A01N59/00A01N65/03A01N25/26A01N25/00A01P7/04A01P19/00C12N1/14C12N3/00C12R1/645
CPCA01N25/00A01N25/26A01N37/36A01N59/00A01N65/00A01N65/03A01N63/30C12N1/14C12N3/00Y02A50/30
Inventor 刘丽娟张本光公茂庆王海防程鹏刘宏美郭秀霞赵玉强王怀位
Owner 山东省寄生虫病防治研究所
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com