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Testing device and testing method for measuring methane oxidation rate of rice field soil

A technology of a test device and a measurement method, which is applied in the field of measuring the methane oxidation rate of paddy field soil, can solve the problems of incapable headspace sampling of gas in the bottle, narrow application range, and poor universality, and achieves wide application range, convenient operation, and reliable results Effect

Inactive Publication Date: 2021-06-04
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method eliminates the influence of methane in the air on the experimental results by using isotope labeling methane, but because it needs to use isotope labeling and is expensive, its application range is narrow and its universality is poor; and because it only has a long needle tube , the gas in the bottle cannot be sampled by headspace

Method used

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  • Testing device and testing method for measuring methane oxidation rate of rice field soil
  • Testing device and testing method for measuring methane oxidation rate of rice field soil
  • Testing device and testing method for measuring methane oxidation rate of rice field soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Such as figure 1 The shown test device for measuring the methane oxidation rate of paddy field soil includes a culture bottle assembly and an air intake assembly and an air extraction assembly that are inserted in parallel on the top of the culture bottle assembly and respectively communicated with the inside of the culture bottle assembly. The bottom of the gas assembly is provided with a gas diffusion assembly.

[0032] Wherein, the culture bottle assembly includes a culture bottle 1 with a top opening and a rubber stopper 2 arranged on the top of the culture bottle 1 and adapted to the top opening of the culture bottle 1, and the air intake component and the air extraction component respectively pass through the rubber stopper from top to bottom. 2.

[0033] The rubber plug 2 is provided with an intake glass tube jack, and the intake assembly includes an intake glass tube 3 that runs through the intake glass tube jack, an intake silicone tube 4 that is arranged on t...

Embodiment 2

[0051] In this embodiment, in the soil-water mixture 13 in the culture bottle 1, the mass of the soil is 40 g, and the mass of the water is 20 g. Measure the methane oxidation rate of rice field soil according to the method in Example 1, collect gas samples on the 1st, 3, 5, 7, 9, and 11 days after the cultivation, and collect the methane concentration in the gas samples obtained by gas chromatography within 3 days The instrument (Agilent 7890N) measured that the concentration of methane in the culture bottle on the 1st, 3rd, 5th, 7th, 9th, and 11th day was 15000ppm, 11800ppm, 10300ppm, 8600ppm, 5600ppm, 3600ppm respectively;

[0052] The methane oxidation rate of paddy soil was calculated according to the following formula:

[0053]

[0054] The methane oxidation rate of paddy field soil was obtained as:

[0055]

[0056] In the formula: F is the methane emission flux, the unit is μg kg -1 h -1 ; ρ is the density of methane under standard conditions, 0.714kg m -3 ; ...

Embodiment 3

[0058] In this embodiment, in the soil-water mixture 13 in the culture bottle 1, the mass of the soil is 50 g, and the mass of the water is 25 g. Measure the methane oxidation rate of rice field soil according to the method in Example 1, collect gas samples on the 1st, 3, 5, 7, 9, and 11 days after the cultivation, and collect the methane concentration in the gas samples obtained by gas chromatography within 3 days The instrument (Agilent 7890N) measured that the concentrations of methane in the culture bottle on the 1st, 3rd, 5th, 7th, 9th, and 11th days were 15000ppm, 10793ppm, 7283ppm, 4391ppm, 2235ppm, and 780ppm respectively;

[0059] The methane oxidation rate of paddy soil was calculated according to the following formula:

[0060]

[0061] The methane oxidation rate of paddy field soil was obtained as:

[0062]

[0063] In the formula: F is the methane emission flux, the unit is μg kg -1 h -1 ; ρ is the density of methane under standard conditions, 0.714kg m ...

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Abstract

The invention relates to a testing device and a testing method for measuring the methane oxidation rate of rice field soil. The testing device comprises a culture bottle assembly, a gas inlet assembly and a gas exhaust assembly, the gas inlet assembly and the gas exhaust assembly are inserted into the top of the culture bottle assembly in parallel and are respectively communicated with the interior of the culture bottle assembly, and a gas diffusion assembly is arranged at the bottom of the gas inlet assembly. The measuring method comprises the following steps: 1) weighing a soil-water mixture and placing the soil-water mixture in a culture bottle assembly; 2) introducing an O2 / N2 gas mixture into the culture bottle assembly through the gas inlet assembly, and exhausting air in the culture bottle assembly through the gas exhaust assembly; 3) injecting methane gas into the culture bottle assembly through the gas inlet assembly, and then closing the gas inlet assembly and the gas exhaust assembly; and 4) placing the culture bottle assembly in an incubator for culture, and regularly sampling for detection. Compared with the prior art, the method has the advantages that an isotopic tracing method is not needed, the oxidation rate of methane can be researched by adopting common pure methane gas, the cost is saved, the application range is wide, and the result is reliable.

Description

technical field [0001] The invention belongs to the technical field of measuring the methane oxidation rate of paddy field soil, and relates to a test device and a measuring method for measuring the methane oxidation rate of paddy field soil. Background technique [0002] my country's rice field methane emissions account for the largest proportion in the world, accounting for about 29.2% of global rice field methane emissions. The emission reduction of rice field methane has been included in the overall emission reduction target of the Chinese government. In the natural environment, soil methane emissions include two major processes: production and oxidation, and these two processes are carried out simultaneously. Therefore, improving methane oxidation efficiency can greatly promote the completion of methane emission reduction tasks in paddy fields. At present, the method of studying the methane oxidation process in rice fields can only be carried out in indoor culture bot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N33/24
CPCG01N30/02G01N33/24G01N33/245
Inventor 方凯凯曹林奎王峻仪小梅戴巍高慧沙之敏
Owner SHANGHAI JIAO TONG UNIV
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