Method for evaluating back fat thickness and eye muscle area of pig and application thereof

A technology for eye muscle area and pig backfat thickness, which is applied in the field of molecular biology, can solve the problems of lack of tools and methods for pig backfat thickness and eye muscle area, and unclear research, so as to improve economic benefits and increase breeding The effect of speed, quickening the pace

Active Publication Date: 2021-06-11
AGRI GENOMICS INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Although a lot of research has been done on the genes related to pig growth traits in the prior art, the molecular mechanism and associated loci of pig backfat th

Method used

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  • Method for evaluating back fat thickness and eye muscle area of pig and application thereof
  • Method for evaluating back fat thickness and eye muscle area of pig and application thereof
  • Method for evaluating back fat thickness and eye muscle area of pig and application thereof

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Example Embodiment

[0038] According to a preferred embodiment of the present invention, the SNP marker located at the reference sequence Chromosome 1:16,077,935 of the International Porcine Genome Version 10.2 has a T / C polymorphism.

[0039] Wherein, the site is located at the 251 bp of the nucleotide fragment shown in SEQ ID NO. 1, and the difference in bases at this site leads to the difference in the thickness of the pig's back fat and the area of ​​the eye muscle.

[0040] In the present invention, the three genotypes corresponding to the SNP site are TT, TC and CC respectively, the TT genotype is that the bases of the SNP marker site in both alleles are T, and the TC genotype is The bases of the SNP marker site in the two alleles are T and C, and the CC genotype is that the bases of the SNP marker site in both alleles are C.

[0041] Preferably, pigs with the genotype TT at the SNP site at Chromosome 1:16,077,935 in the reference sequence of the International Porcine Genome Version 10.2 ha...

Example Embodiment

[0113] Example 1

[0114] 1. Experimental animals

[0115] The experimental pig population used in this example is Yorkshire purebred pigs from Hengshui Breeding Pig Farm, Hebei Province, with a total of 384 pigs, including 27 boars and 357 sows.

[0116] 2. Measurement and correction of eye muscle area

[0117] When the body weight of each pig is in the range of 85-105kg, the eye muscle area and body weight of the individual are measured, and the data such as the measurement day and age are recorded. The collected data were corrected for phenotypic data using the genetic evaluation trait determination procedures of the Hebei Provincial Local Standard (DB 13 / T 2065-2014) document "Technical Regulations for Determination of Production Performance in Breeding Farms". At the same time, the pig ear tissue samples of the experimental pig population were collected, placed in PBS buffer, and stored at low temperature.

[0118] When the body weight was measured, the area of ​​the ...

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Abstract

The invention discloses an SNP (Single Nucleotide Polymorphism) marker for evaluating the back fat thickness and the eye muscle area of a pig as well as an recognition method and application of the SNP marker, and the SNP marker is located on an ENSSSCG00000004081 gene, is located at reference sequences Chromosome1: 16, 077 and 935 of an international pig genome version 10.2, and has T/C polymorphism. The determination of the SNP marker related to evaluation of pig backfat thickness and 100kg ocular muscle area can assist in selection of pig dominant varieties with low backfat thickness and high ocular muscle area, shortens the breeding period, and improves the breeding efficiency and breeding precision.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a SNP marker, method and application for evaluating pig backfat thickness and eye muscle area. Background technique [0002] In the pig breeding process, production performance has always been an important factor in the feeding process, which is directly related to the economic benefits of the entire pig industry. Pig backfat thickness and eye muscle area are quantitative traits, which have high breeding value in pig breeding. Therefore, it is necessary to conduct an in-depth study on the molecular mechanism of pig backfat thickness and eye muscle area traits, so as to provide a basis for pig genome breeding. [0003] Micro-effect polygene refers to a type of gene that has a large number of genes that affect the phenotype, but the effect of each gene is small, and their individual effects cannot be distinguished. Minor effect polygenes are mainly related t...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6809C12N15/11G16B20/20G16B20/30
CPCC12Q1/6888C12Q1/6809G16B20/20G16B20/30C12Q2600/124C12Q2600/156C12Q2600/172C12Q2531/113C12Q2521/525C12Q2535/125C12Q2565/627Y02P60/87
Inventor 唐中林陈云易国强杨亚岚
Owner AGRI GENOMICS INST CHINESE ACADEMY OF AGRI SCI
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