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Bacillus siamensis for preventing and treating juglans regia fruit dropping disease and application thereof

A technology of bacillus and walnut, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of endangering the health and safety of humans and animals and the ecological environment, enhancing the drug resistance of pathogenic microorganisms, reducing the control effect, etc., achieving non-toxic side effects, Strong inhibitory effect, high content of live bacteria

Active Publication Date: 2021-06-15
SICHUAN ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] One of purpose of the present invention is to solve above-mentioned deficiency, provide a bacillus and application of preventing and treating walnut fruit drop disease, in order to solve the existing problem of preventing and treating walnut by chemical agents This disease will not only enhance the drug resistance of pathogenic microorganisms and reduce the control effect, but also cause serious pollution to the environment and endanger the health and safety of humans and animals and the ecological environment.

Method used

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  • Bacillus siamensis for preventing and treating juglans regia fruit dropping disease and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Isolation, Identification and Preservation of Strain CT04

[0020] of the present invention Bacillus siamensis Strain CT04 was isolated from the soil of Zengjia hickory planting area in Chaotian District, Guangyuan City, Sichuan Province by soil dilution separation method.

[0021] 1. Separation

[0022] (1) Soil sample collection: Random sampling in the walnut planting area, sampling from the soil surface to 15cm, 200g from each place, passing through a coarse sieve, and taking 10g for separation;

[0023] (2) Plate preparation: Melt the sterilized LB solid medium, pour it into a sterilized petri dish, cool down, make a plate, and set aside;

[0024] (3) Weigh 10g of soil sample into a sterilized Erlenmeyer flask, add 90ml of sterile water, shake on a shaker at 170r / min for 5min to make a soil suspension, let it stand for 10min, take the supernatant, and rinse with double distilled water diluted to 10 6 cfu / mL.

[0025] (4) Take 100 µl of the above soil suspensio...

Embodiment 2

[0036] Validation of indoor effect of Bacillus strain CT04 on walnut fruit drop disease

[0037] 1. Experimental method

[0038] Using the flat plate confrontation method, the tested walnut fruit drop pathogens (the pathogenic bacteria of walnut anthracnose and the pathogenic bacteria of walnut brown spot disease) were respectively punched with a sterile puncher, and then transferred to the PDA solid medium, and the pathogenic bacteria cake was placed Inoculate Bacillus in the center of the plate, 2cm around Bacillus siamensis Strain CT04 was cultured in a biochemical incubator at 28°C, each treatment was repeated 3 times, and the blank LB was used as a control; when the control hyphae covered the plate, the diameter of the colony and the diameter of the inhibition zone were measured by the cross method, and the average value was calculated and antibacterial rate.

[0039] Inhibitory rate (%) = (control colony diameter - treated colony diameter) / control colony diameter × 1...

Embodiment 3

[0045] A preparation method of a bacillus bacterial agent for preventing and treating walnut fruit drop, comprising the following steps, step a, culturing the bacillus bacterial strain on LB solid medium, and the mass of each component in the LB solid medium is respectively, NaCl6~ 8g, 4~6g of yeast extract, 8~12g of tryptone, 16~24g of agar powder, distilled water to 1L, pH value 7.0, and streak culture, culture temperature 30 ℃, culture time 24h; steps b. Pick a single colony and inoculate it in LB liquid medium for cultivation. The components in LB liquid medium are as follows: 6-8g NaCl, 4-6g yeast extract, 8-12g tryptone, and distilled water to volume to 1L, pH value 7.0, temperature 30°C, rotation speed 160r / min shaking culture, culture 20h; step c, centrifuge the culture product prepared by step b at 8000 r / min for 5min, let it stand for 5-10min, and Abandon the supernatant, and the resulting precipitate is bacillus thalline; step d, dilute the bacillus thalline prepare...

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Abstract

The invention discloses bacillus siamensis for preventing and treating juglans regia fruit dropping disease and application thereof. The bacillus siamensis is a bacillus siamensis strain CT04 and is preserved in China General Microbiological Culture Collection Center (CGMCC) on June 14, 2018, and the preservation number is CGMCC No.15943. The bacillus siamensis is expected to solve the problems that when the juglans regia fruit dropping disease is prevented and treated through chemical agents in the prior art, the drug resistance of pathogenic microorganisms can be enhanced, the control effect is reduced, the serious pollution to the environment is caused, and the health and safety of people and livestock and the ecological environment are endangered.

Description

technical field [0001] The invention relates to the field of microorganism application, more specifically, the invention mainly relates to a bacillus strain for preventing and treating walnut fruit drop disease and its application. Background technique [0002] Walnut (Juglans regis L) is one of the four major dried fruits in the world. The fruit is rich in nutrients and contains various trace elements and unsaturated fatty acids needed by the human body. Sichuan Province is a large walnut producing province in my country, with extremely rich resources and its output ranks second in the country. In recent years, Sichuan's nuclear industry has developed rapidly. The province's walnut cultivation area and output have grown from 70,000 mu and an annual output of 606 tons in the 1950s to 975,000 mu and an annual output of 29,834.39 tons in 2000. By the end of 2016, the walnut planting area had reached 195 million mu. However, the occurrence of walnut diseases is also showing a...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/07
CPCC12N1/20A01N63/22
Inventor 杨莉王新杨育林杨双昱杨远亮
Owner SICHUAN ACAD OF FORESTRY
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