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Pantoea agglomerans and application thereof in prevention and treatment of pepper diseases

A kind of technology of agglomeration of Pantoea and Zanthoxylum bungeanum

Active Publication Date: 2022-07-26
SHANDONG FOREST SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the use of Pantoea agglomerans to control pepper canker

Method used

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  • Pantoea agglomerans and application thereof in prevention and treatment of pepper diseases
  • Pantoea agglomerans and application thereof in prevention and treatment of pepper diseases
  • Pantoea agglomerans and application thereof in prevention and treatment of pepper diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Isolation and identification of P. agglomerans HJEB-01

[0017] 1. Separation:

[0018] The pantoea agglomerans of the present invention is obtained from the prickly ash plantation in Heze City, Shandong Province by using the tissue separation method, and the separation method is as follows:

[0019] (1) Tissue collection: collect healthy and disease-free branches of Chinese prickly ash from the prickly ash orchard with a severe incidence of prickly ash, put it in a sealed bag, and bring it back to the laboratory for use;

[0020] (2) medium plate preparation: potato dextrose agar medium (PDA) is melted, poured into sterile petri dish respectively, cooled, and made PDA plate;

[0021] (3) Carry out the following treatments in turn on the recovered healthy Chinese prickly ash branches: 1. Rinse with running tap water for 1 min and air dry; 2. Use 75% ethanol for surface disinfection; 3. Under aseptic conditions, use sterile Cut the prickly ash branches into ...

Embodiment 2

[0034] Example 2 Indoor effect verification of Pantoea agglomerates HJEB-01 on prickly ash canker

[0035] 1. Experimental method

[0036] Using the plate confrontation culture method, the tested prickly ash (Diaporthe eres) bacterial cake (9mm) was moved to the center of the plate, and the HJEB-01 strain was symmetrically inoculated at a distance of 2 cm from the prickly pear canker bacterial cake, and cultured in a biochemical incubator at 28 °C. , and each treatment was repeated 3 times. When the hyphae of the control group (only inoculated with 9mm prickly ash canker cake in the center of the plate) covered the plate, the colony diameter and the diameter of the inhibition zone were measured by the cross method, and the average value and relative inhibition rate were calculated.

[0037] 2. Results

[0038] Depend on figure 2It can be seen from Table 2 that HJEB-01 has a strong inhibitory effect on the mycelium growth of prickly ash, the diameter of the antibacterial zo...

Embodiment 3

[0041] Example 3 Preparation of P. agglomerans HJEB-01 fermentation broth

[0042] Seed liquid medium: beef extract 3g, peptone 5g, sodium chloride 5g, water 1000mL, pH 7.3.

[0043] Fermentation medium formula (weight percentage): peptone 0.5%, yeast extract 0.5%, sucrose 1%, soybean flour 2.5%, corn flour 1%, light calcium carbonate 0.25%, sodium chloride 0.05%, the rest is water , pH 8.

[0044] Preparation:

[0045] 1) Seed liquid culture: Pick a small amount of colonies from the slanted surface of the test tube for Pantoea agglomerans strain HJEB-01, move it to the seed liquid medium, and shake it at 30°C for 24-36 hours, which is the seed liquid;

[0046] 2) Fermentation and culture conditions: culture temperature of 30°C, initial pH of 8, liquid filling volume of 70%, bacterial inoculation volume of 1.5%, culture time of 48h, ventilation volume of 25mL / 250mL, rotation speed of 200r / min, using this medium for fermentation and culture, viable bacteria The number can re...

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Abstract

The invention discloses pantoea agglomerans and application of the pantoea agglomerans to prevention and treatment of pepper diseases. The Pantoea agglomerans HJEB-01 disclosed by the invention is separated from pepper branches by adopting a tissue isolation method, and the preservation number of the Pantoea agglomerans HJEB-01 is CCTCC (China Center for Type Culture Collection) No: M 2022255. The pantoea agglomerans disclosed by the invention has the characteristics of novel strains, high efficiency and high content of living bacteria, 100 times of fermentation liquor of the pantoea agglomerans has a remarkable effect of preventing and treating the pepper canker, the prevention and treatment effect reaches 63.45%, and no phytotoxicity is generated. Meanwhile, the composition is safe, efficient, free of pesticide residues and toxic and side effects and capable of reducing use of chemical pesticides, and therefore the composition has important application prospects in biological prevention and treatment of pepper diseases such as pepper canker.

Description

technical field [0001] The invention belongs to the field of application of microorganisms, and in particular, the invention relates to a strain of Pantoea agglomerans and uses thereof. Background technique [0002] Zanthoxylum bungeanum, native to China, is a small deciduous tree or shrub, belonging to the family Rutaceae and the genus Zanthoxylum. Zanthoxylum bungeanum is resistant to drought and barrenness, has strong suitability and is widely distributed. It is an important economic tree species in my country. Zanthoxylum bungeanum is an important edible condiment, Chinese medicinal material, spice and woody oil tree species. At the same time, the root system of Zanthoxylum bungeanum is developed to prevent soil erosion. Therefore, the planting of Zanthoxylum bungeanum has important economic and ecological value and plays an important role in the revitalization of local villages. my country is a big country in the production and trade of prickly ash, with a planting are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/20A01P3/00C12R1/01
CPCC12N1/20A01N63/20Y02A50/30
Inventor 王清海亓玉昆刘在哲吕娟王俊伟韩新英杨静朱文成孔令刚刘慇万平平
Owner SHANDONG FOREST SCI RES INST
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