Fuco-oligosaccharide with anti-hyperuricemia activity and preparation method and application thereof

A technology of hyperuricemia and fucoid oligosaccharide, which is applied in the direction of organic active ingredients, medical preparations containing active ingredients, and pharmaceutical formulations, etc., can solve the problems of producing harmful by-products and uncontrollable degradation process.

Active Publication Date: 2021-06-18
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, at present, the preparation of fucoidal oligosaccharides usually adopts physical and chemical degradation methods, which have the disad

Method used

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  • Fuco-oligosaccharide with anti-hyperuricemia activity and preparation method and application thereof
  • Fuco-oligosaccharide with anti-hyperuricemia activity and preparation method and application thereof
  • Fuco-oligosaccharide with anti-hyperuricemia activity and preparation method and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0043] Preparation of fucoidanase

[0044] (1) Enzyme-producing microorganisms

[0045] The present invention firstly prepares fucoidanase, the microorganism used is Flavobacteriaceaesp. RC2-3mut, the colony of the bacterial strain RC2-3mut is yellow, round and raised, with neat edges, no movement, cell Gram staining Negative, catalase negative, oxidase positive, grown in seawater, grown in 1% NaCl.

[0046] (2) Enzyme production method of RC2-3mut

[0047] Inoculate the microorganism Flavobacteriaceae sp. RC2-3mut strain in a liquid medium, and culture it on a shaker at 180-250 rpm at 30-35°C for 12-24 hours to obtain a seed liquid; then inoculate the seed liquid with 10% inoculum amount In the fermentation medium, ferment in a 5L fermenter (loading 3-4L), culture at 30-35°C, 150-200rpm for 24-72h; centrifuge the fermentation broth at 10,000rpm at 4°C for 5min, discard the supernatant and take the precipitate , and then use 5 times the volume of 20mM Tris-HCl solution with...

Embodiment 1

[0051] The enzymatic hydrolysis preparation method of embodiment 1 fucoidal oligosaccharide

[0052] This embodiment provides an enzymatic preparation method of fucoidal oligosaccharides, which includes the following steps:

[0053] (1) Configuration of the fucoidan solution: the buffer solution is 24mM PBS, the pH value is 8.0, or 24mM Tris-HCl is selected, the pH value is 8.0, and the fucoidan is dissolved in the above buffer solution to make 0.1% - 1% solution of fucoidan.

[0054] (2) Fucoidanase configuration: the buffer solution is 24mM PBS, pH 8.0, or 24mM Tris-HCl, pH 8.0. Dissolving the fucoidanase prepared according to the above-mentioned method in the above buffer solution, it is configured so that the activity concentration is 500-1000 U / mL.

[0055] (3) Preparation of fucoidan oligosaccharides by enzymatic method: add 1 mL of 500 U / mL fucoidan enzyme to 10 mL of 0.1%-10% fucoidan solution, and put it in a water bath at 50 to 180 r / min under the condition of 25 t...

Embodiment 2

[0082] Example 2 Application of fucoidal oligosaccharides in inhibiting xanthine oxidase

[0083] 1. This example provides an application of fucoidan oligosaccharide prepared by enzymatic hydrolysis to inhibit xanthine oxidase in vitro.

[0084] In this example, based on the principle of inhibiting the activity of xanthine oxidase and inhibiting the production of uric acid, the following experiments were set up to detect the xanthine oxidase inhibitory performance of different fucoidal oligosaccharides. The method is as follows:

[0085] (1) The fucoidan oligosaccharides of different molecular weights prepared by the biological enzymatic hydrolysis method in Example 1 were respectively dissolved in distilled water, and finally a solution with a concentration of 0.1%-1% was prepared.

[0086] (2) Xanthine was dissolved in PBS with a concentration of 0.05M and a pH value of 7.4, and the solution was prepared to have a final concentration of 0.5mM.

[0087] (3) Prepare xanthine ...

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Abstract

The invention discloses fuco-oligosaccharide with anti-hyperuricemia activity and a preparation method and application thereof. The preparation method prepares the fuco-oligosaccharide with anti-hyperuricemia activity by using fucoidanase to degrade fucoidin. The fuco-oligosaccharide prepared by the method can be applied to prepare hyperuricemia-inhibiting and anti-gout products. The provided preparation method of the fuco-oligosaccharide adopts biological enzymic degradation; the preparation method is mild in degradation and controllable in process; harmful by-products such as chemicals cannot be generated during degradation; and the obtained fuco-oligosaccharide is safe and environment-friendly. The fuco-oligosaccharide has anti-hyperuricemia activity, can effectively control the synthesis of uric acid and can also effectively reduce the content of creatinine and urea nitrogen in bodies, and therefore renal functions can be effectively protected.

Description

technical field [0001] The invention relates to the technical field of fucoid oligosaccharides, in particular to a fuco oligosaccharide with anti-hyperuricemia activity and a preparation method and application thereof. Background technique [0002] Hyperuricemia is due to the long-term blockage of purine metabolism, resulting in blood uric acid levels higher than the normal range (blood uric acid levels ≥ 416μmol / L), and can cause gout, a heterogeneous disease that causes tissue damage. The clinical manifestations of gout are joint pain, deformation and easy involvement of the kidneys and cardiovascular system. At the same time, hyperuricemia is significantly positively correlated with obesity, hyperlipidemia, hypertension, diabetes, atherosclerosis and other diseases. Therefore, hyperuricemia is a serious metabolic disease that endangers human health. [0003] In recent years, with the improvement of people's living standards and changes in dietary structure, the intake o...

Claims

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Application Information

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IPC IPC(8): A61K31/715A61K31/702A61P19/06A23L33/125A23L2/38C12P19/00C12P19/14
CPCA61K31/715A61K31/702A61P19/06A23L33/125A23L2/38C12P19/00C12P19/14A23V2002/00A23V2200/30
Inventor 王莹陈芊汝李昀牛德军陈铁军朱昱兴
Owner QINGDAO AGRI UNIV
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