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Preparation method and application of potentiometric microelectrode for in-situ detection of cerebrospinal fluid calcium ions

An in-situ detection and micro-electrode technology, applied in applications, measurement devices, blood characterization devices, etc., can solve the problems of lack of temporal and spatial resolution, inability to realize in-situ monitoring of cerebrospinal fluid extracellular ions, etc., to improve electrode response performance, The effect of high mechanical strength and strong anti-interference ability

Pending Publication Date: 2021-06-18
BINZHOU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Both the patch clamp electrophysiological technique and the fluorescence detection method have the technical difficulty of in situ monitoring of extracellular ions in the cerebrospinal fluid due to the lack of sufficient temporal and spatial resolution.

Method used

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  • Preparation method and application of potentiometric microelectrode for in-situ detection of cerebrospinal fluid calcium ions
  • Preparation method and application of potentiometric microelectrode for in-situ detection of cerebrospinal fluid calcium ions
  • Preparation method and application of potentiometric microelectrode for in-situ detection of cerebrospinal fluid calcium ions

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Embodiment

[0053] This embodiment relates to a preparation method of a potentiometric microelectrode for in-situ detection of cerebrospinal fluid calcium ions, comprising the following steps:

[0054] Step 1, making acupuncture needle microelectrodes;

[0055] Step 2, surface modification of acupuncture needle microelectrodes to obtain acupuncture needle microelectrodes with deposited PEDOT / PSS conductive layer;

[0056] Step 3, covering the calcium ion-selective membrane solution on the surface of the acupuncture needle microelectrode on which the PEDOT / PSS conductive layer is deposited by dipping method, and then obtaining the polymer membrane ion-selective acupuncture needle microelectrode modified by PEDOT / PSS;

[0057] Step 4, measure the potential response of the ion-selective acupuncture needle microelectrode in the calcium chloride solution, and determine the linear response range and detection life of the electrode to calcium ions.

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Abstract

The invention provides a preparation method and application of a potentiometric microelectrode for in-situ detection of cerebrospinal fluid calcium ions. The polymer membrane ion selective microelectrode mainly comprises a silver-plated acupuncture needle, a PEDOT(PSS) conducting layer and a polymer ion selective membrane, the ion selective membrane can specifically measure the ions according to the types of ion carriers in the membrane, the electron transfer rate can be improved by modifying the solid conducting layer, the electrode reaction performance is improved, and the electrochemical principle is a Nernst equation, namely, the change of electrode potential is in direct proportion to the logarithm of the concentration of the chemical component. The potentiometric microelectrode measurement is realized through an ion selective membrane, and when the microelectrode is balanced with a solution, a potential difference is formed between two sides of the membrane due to the ion effect.

Description

technical field [0001] The invention belongs to the field of electrode detection; in particular, it relates to a preparation method and application of a potentiometric microelectrode for in-situ detection of cerebrospinal fluid calcium ions. Background technique [0002] Cerebrospinal fluid provides a stable ecological environment for the brain and spinal cord, in which changes in ion content play a very important role in the maintenance and function of neurons. Calcium ion is an important second messenger in the cell and an important factor for maintaining the function of neuron cells. The imbalance of intracellular calcium ion content is one of the pathogenesis of neuron cell damage. The content of intracellular calcium ions is directly related to the concentration of calcium ions in cerebrospinal fluid. Therefore, in situ monitoring of calcium ions in cerebrospinal fluid is of great significance for the study of neuronal cell physiological and pathological functions. [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/333A61B5/1473A61B5/145C25D9/02C25D7/00
CPCG01N27/333A61B5/1473A61B5/14546C25D9/02C25D7/00
Inventor 赵光涛翟佳丽
Owner BINZHOU MEDICAL COLLEGE
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