Recombinant plasmid, construction method thereof, recombinant image system and application

Abstract

The invention discloses a recombinant plasmid, a construction method thereof, a recombinant image system and application. According to the invention, aiming at the protein-protein interaction of MSI1 / AGO2, the qualitative and quantitative reliability of the biooptics-fluorescent resonance energy transfer technology and the luciferase separation and complementation system and the optimization of the luciferase separation and complementation system are discussed. According to the invention, through testing linker peptides with different lengths and elasticity / charge distribution, a group of recombinant image systems of Gaussian luciferase (GLuc) of which the brightness is thousands of times higher than that of firefly luciferase (FLLuc) are successfully constructed; meanwhile, the firefly luciferase is used as a standardized signal during quantitative analysis. The recombinant plasmid is mainly applied to preclinical cell experiments and animal experiments, and the treatment effect of the brain tumor can be measured and quantified in real time and in a non-invasive mode.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a recombinant plasmid and its construction method, a recombinant imaging system and its application. Background technique [0002] Now that the visualization of malignant tumors has entered the 21st century, no matter in terms of animal models, drug pre-development, pharmacokinetics, and even sequential treatment plan decisions, etc., it has already occupied a large volume of scientific research in biomedicine . Molecular imaging is a science aimed at the visualization, realization and quantification of molecular mechanisms. Use a variety of molecular design and imaging platforms to achieve non-invasive analysis. Since the 1960s, the green fluorescent protein was isolated and defined by the Japanese scholar Osamu Shimomura, and various imaging technologies using optical principles have emerged as the times require. With the improvement and development of green fluorescent ...

AI Technical Summary

Problems solved by technology

In the aforementioned studies, Paulmurugan and Gambhir et al. conducted several different protein truncation experiments according to the position of the catalytic domain of Renilla, and used a common neutral linker peptide (GGGGS) between the truncated Renilla luciferase and the tested protein MyoD 2 However, whether the presence of linking peptides affects the complementarity of luciferase remains to be studied

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