Flower color regulatory gene MaGT, its application and method for quickly verifying its function

A technology for regulating genes and flower colors, applied in application, genetic engineering, plant genetic improvement, etc., can solve the problems of grape hyacinth flowers without anthocyanin 3-O-glucosyltransferase gene, and speed up gene function research The effect of the process

Pending Publication Date: 2021-06-29
NORTHWEST A & F UNIV
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, no one has applied the occurrence pathway of grape hyacinth perianth slices to the acquisition of transgenic flowers and rapid functional evaluati...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Flower color regulatory gene MaGT, its application and method for quickly verifying its function
  • Flower color regulatory gene MaGT, its application and method for quickly verifying its function
  • Flower color regulatory gene MaGT, its application and method for quickly verifying its function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1. Method for quickly verifying the function of the flower color regulation gene MaGT

[0026] 1. Plant material

[0027] The five-year-old bulbs of grape hyacinth Armenia (M. armeniacum) were purchased from Zhejiang Hongyue Seed Industry Co., Ltd. and planted in the experimental field of Northwest Agriculture and Forestry University in Yangling Demonstration Area, Shaanxi Province.

[0028] 2. Regeneration of tepals of grape hyacinth in vitro

[0029] Respectively from the young and mature inflorescences of grape hyacinth ( figure 1 c) The small flower bud explants collected from 4 different developmental stages ( figure 1aI-IV), the surface of the explants was sterilized in 75% (v / v) ethanol for 30 seconds, degermed with 0.1% HgCl2 for 5 minutes, and washed twice with sterile water. Add 3% (w / v) sucrose, 0.3% (w / v) plant gel, 0.45μM 2,4-dichlorophenoxyacetic acid (2,4-dichlorophenoxyacetic acid, 2,4-D) and different concentrations of 6-benzyladenine (6-ben...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of plant molecular biology, and particularly relates to a flower color regulatory gene MaGT, its application and a method for quickly verifying its function. The nucleotide sequence of the flower color regulatory gene MaGT is shown as SEQ ID NO: 1. The method comprises the following steps: S1, placing grape hyacinth flower buds in a color changing period on an MS culture medium added with 0.45 [mu] M of 2, 4-dichlorophenoxyacetic acid and 8.88 [mu] M of 6-benzyladenine for continuous culture until a tepal is regenerated; S2, introducing a GUS gene into the grape hyacinth tepal, and screening the grape hyacinth tepal carrying and stably expressing the exogenous GUS gene; and S3, integrating an RNAi interference vector of MaGT into the grape hyacinth tepal obtained in the S2 to obtain a transgenic grape hyacinth tepal, and comparing the transgenic grape hyacinth tepal with a non-transgenic control flower color phenotype to determine the regulation function of the MaGT gene on the flower color. The MaGT gene disclosed by the invention can be used for breeding grape hyacinth, a new variety with light flower color can be cultivated, and the method for quickly verifying the function of the MaGT gene can accelerate the gene function research process of flowers.

Description

technical field [0001] The invention belongs to the technical field of plant molecular biology, and in particular relates to a flower color regulating gene MaGT, its application and a method for rapidly verifying its function. Background technique [0002] Grape hyacinth (Muscari spp.) is an important ornamental flower bulb known for its rare blue flower color and musky smell. It is widely used in flower beds, flower mirrors and garden potted plants. Rapid developments in the fields of genomics and bioinformatics have greatly advanced our understanding of important agronomic traits in flower tissues. However, these massive sequence and expression information cannot fully reveal gene functions, and overexpressing and inhibiting target genes in native plants is still an important strategy for functional research. However, the existing transgenic system of grape hyacinth is cumbersome and difficult, and the childhood period is long (3–5 years), which greatly limits the effici...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/54C12N9/10C12N15/84C12N1/21A01H5/02A01H6/56
CPCC12N9/1051C12N15/8218C12N15/825C12N15/8212C12N15/8205C12Y204/01238
Inventor 娄倩刘红利罗文
Owner NORTHWEST A & F UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap