Flower color regulatory gene MaGT, its application and method for quickly verifying its function

Abstract

The invention belongs to the technical field of plant molecular biology, and particularly relates to a flower color regulatory gene MaGT, its application and a method for quickly verifying its function. The nucleotide sequence of the flower color regulatory gene MaGT is shown as SEQ ID NO: 1. The method comprises the following steps: S1, placing grape hyacinth flower buds in a color changing period on an MS culture medium added with 0.45 [mu] M of 2, 4-dichlorophenoxyacetic acid and 8.88 [mu] M of 6-benzyladenine for continuous culture until a tepal is regenerated; S2, introducing a GUS gene into the grape hyacinth tepal, and screening the grape hyacinth tepal carrying and stably expressing the exogenous GUS gene; and S3, integrating an RNAi interference vector of MaGT into the grape hyacinth tepal obtained in the S2 to obtain a transgenic grape hyacinth tepal, and comparing the transgenic grape hyacinth tepal with a non-transgenic control flower color phenotype to determine the regulation function of the MaGT gene on the flower color. The MaGT gene disclosed by the invention can be used for breeding grape hyacinth, a new variety with light flower color can be cultivated, and the method for quickly verifying the function of the MaGT gene can accelerate the gene function research process of flowers.

Description

technical field [0001] The invention belongs to the technical field of plant molecular biology, and in particular relates to a flower color regulating gene MaGT, its application and a method for rapidly verifying its function. Background technique [0002] Grape hyacinth (Muscari spp.) is an important ornamental flower bulb known for its rare blue flower color and musky smell. It is widely used in flower beds, flower mirrors and garden potted plants. Rapid developments in the fields of genomics and bioinformatics have greatly advanced our understanding of important agronomic traits in flower tissues. However, these massive sequence and expression information cannot fully reveal gene functions, and overexpressing and inhibiting target genes in native plants is still an important strategy for functional research. However, the existing transgenic system of grape hyacinth is cumbersome and difficult, and the childhood period is long (3–5 years), which greatly limits the effici...

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Problems solved by technology

[0005] However, no one has applied the occurrence pathway of grape hyacinth perianth slices to the acquisition of transgenic flowers and rapid functional evaluation of exogenous genes, and no anthocyanin 3-O-glucosyltransferase gene (MaGT) has the ability to regulate grape hyacinth. Report on Suit and Color Function of Hyacinth

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