Molecular markers for screening soybean materials, screening method, breeding method and application

Abstract

The invention discloses molecular markers for screening soybean materials, a screening method, a breeding method and application, the molecular marker comprises an InDel marker or / and a dCAPS marker, and the method for screening the soybean high-protein materials by using the molecular markers comprises the following steps: selecting a primer pair of the InDel marker; selecting DNA of one or more soybean varieties to carry out first PCR amplification by utilizing the primer pair of the InDel marker; and performing first electrophoresis detection on the first PCR amplification product. Soybean varieties with high bands in the first electrophoresis detection band are low-protein varieties, and soybean varieties with low bands in the first electrophoresis detection band are high-protein varieties. Based on whole genome re-sequencing and QTL positioning technologies, high-protein InDel and dCAPS with definite targeting are developed, and the combination of the InDel and dCAPS markers provides a new way for screening of soybean high-protein resources and molecular marker-assisted breeding of high-protein breeding materials or varieties with excellent agronomic and quality traits in the future.

Description

technical field [0001] The invention belongs to the field of crop genetics and breeding, and relates to a molecular marker for screening soybean high-protein materials, a method for screening soybean high-protein materials using the primers, and a method for using the primers to screen target strains for breeding. Background technique [0002] Soybean grains are rich in protein, accounting for 31% to 55% of grain dry matter, and are one of the main sources of human food protein. Its protein output accounts for two-thirds of the protein output of protein crops, and what is even more rare is that it is rich in a variety of essential amino acids. The World Health Organization defines soybean protein as a first-class protein. At the same time, soybean protein has good functional properties and can be used in a variety of food systems to improve product quality. [0003] At present, protein-related QTL loci have been found on all 20 chromosomes of soybean, among which 213 protei...

AI Technical Summary

Problems solved by technology

[0006] Most of the currently developed molecular markers are based on linkage analysis and cannot directly target the target gene. The separation and recombination of genes will cause the loss of marker information and cause certain difficulties in the selection of target traits.

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