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Lipid droplet targeting carbon dot, preparation method and application

A technology of carbon dots and lipid droplets, applied in chemical instruments and methods, nanocarbons, fluorescence/phosphorescence, etc., can solve problems such as limiting the in-depth study of carbon dots, and achieve large Stokes shift, high signal-to-noise ratio, light stable effect

Active Publication Date: 2022-06-07
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few reports on lipid droplet-specific probes based on carbon dots, which greatly limits the in-depth study of carbon dots in the field of bioimaging.

Method used

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  • Lipid droplet targeting carbon dot, preparation method and application
  • Lipid droplet targeting carbon dot, preparation method and application
  • Lipid droplet targeting carbon dot, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The preparation method of carbon dots comprises the following steps:

[0049] First, 50 mg of 4-(1-piperidinyl)aniline was weighed and dissolved in 5 mL of absolute ethanol, then the solution was transferred to a polytetrafluoroethylene-lined autoclave, and the autoclave was placed in an oven. In a dry box, heated to 160 °C and reacted for 12 h. After the reaction was completed, the reaction kettle was cooled to room temperature to obtain a brown-red solution, and then the crude product was purified by silica gel column chromatography, using petroleum ether and ethyl acetate in different proportions as eluents, through rotary evaporation and further drying, Obtain purified fluorescent carbon dots.

Embodiment 2

[0055] Fluorescence intensity changes of carbon dots in different solvents

[0056] Add 2 mL of different solvents to the cuvette, add 4 μL of the carbon dots stock solution, and measure the fluorescence profile. The experimental results show that: figure 1 As shown, the fluorescence of carbon dots in aqueous solution is very weak and negligible, and has the potential for wash-free cell imaging.

Embodiment 3

[0058] Fluorescence intensity changes in 1,4-dioxane solutions with different water contents (0-100%)

[0059] Add 2 mL of 1,4-dioxane solutions with different water contents (0-100%) and 4 μL of carbon dots stock solution to the cuvettes and measure the fluorescence spectra. The experimental results show that: figure 2 As shown, the fluorescence of Cdots in pure 1,4-dioxane solution is very strong, which is favorable for Cdots to target lipid droplets. Moreover, the fluorescence of carbon dots in aqueous solution is very weak and negligible, which has the potential for no-wash lipid droplet imaging.

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Abstract

The invention provides a lipid droplet-targeted carbon dot, its preparation method and application. The fluorescent carbon dot uses 4-(1-piperidinyl)aniline as a raw material and absolute ethanol as a solvent, and reacts in a high-pressure reactor at 160°C for 12 h, the crude product after reaction was purified by silica gel column chromatography, using different proportions of petroleum ether and ethyl acetate as eluent, rotary evaporation and further drying to obtain purified fluorescent carbon dots. The carbon dots can rapidly permeate the cell membrane and accumulate in lipid droplets within 10 s, which can be used for ultrafast wash-free lipid droplet imaging in living cells and the changes of non-alcoholic fatty liver caused by bisphenol A. The synthesis and purification of the fluorescent carbon dots of the present invention are very simple, and have the advantages of good light stability, large Stokes shift, high signal-to-noise ratio, etc., can quickly enter cells, and can naturally target lipid droplets.

Description

technical field [0001] The invention relates to the field of fluorescent nanomaterial manufacturing, in particular to a lipid droplet targeting carbon point, a preparation method and an application. Background technique [0002] Lipid droplets are dynamic spherical organelles composed of a hydrophobic core and a phospholipid monolayer composed of various neutral lipids (such as triglycerides and cholesteryl esters), which play an integral role in various physiological processes, such as: Lipid metabolism and storage, signal transduction, apoptosis, protein degradation, etc. The abnormal accumulation of lipid droplets can lead to the risk of fatty liver, obesity, diabetes, and even cancer. Therefore, the imaging of lipid droplets is very necessary, which is very meaningful for studying the function of lipid droplets under normal or physiological and pathological conditions, and also provides useful information for early clinical diagnosis of lipid droplet-related diseases. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/65C01B32/15G01N21/64B82Y20/00B82Y40/00
CPCC09K11/65G01N21/6428C01B32/15B82Y20/00B82Y40/00
Inventor 李朝辉王军丽孙远强杨冉屈凌波
Owner ZHENGZHOU UNIV
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