Establishment method of sciatic nerve injury model

A technology of sciatic nerve and establishment method, which is applied in the research field of nerve injury disease, and can solve the problems of destroying nerve cells and high construction difficulty

Pending Publication Date: 2021-07-13
佛山市第二人民医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The isolated cell injury model can only understand the changes of the model subjects through analysis and testing methods; animal models are easier to observe the changes of the subjects, but because the physical intervention method requires additional fixtures or the need to directly destroy nerve cells, Has high construction difficulty

Method used

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  • Establishment method of sciatic nerve injury model
  • Establishment method of sciatic nerve injury model
  • Establishment method of sciatic nerve injury model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1.1 Experimental materials and animal groups

[0027] Two-month-old male C57 mice were selected, weighing 20-30 g, fed at room temperature (23±2)°C, light control for 12 hours (7:00am to 7:00pm), and free access to water. 1% ropivacaine hydrochloride was purchased from AstraZeneca AB, Sweden.

[0028] 1.2 Experimental grouping

[0029] The experiment was divided into 3 groups, namely normal group (n=3), sham operation group and ropivacaine group (n=3).

[0030] This experimental study complied with the standard requirements of the Animal Protection Committee, and the animals were anesthetized during the experiment to minimize the pain of the animals.

[0031] 1.3 Preoperative preparation

[0032] The motor nerve function of the mice in each group was scored before operation, and those with limb dysfunction were excluded. And Von-Frey method was used to measure the mechanical leg withdrawal threshold of mice, which was used for postoperative control.

[0033] 1.4 Mo...

Embodiment 2

[0040] 2.1 Experimental materials and animal groups

[0041] Two-month-old male C57 mice were selected, weighing 20-30 g, fed at room temperature (23±2)°C, light control for 12 hours (7:00am to 7:00pm), and free access to water. 1% ropivacaine hydrochloride was purchased from AstraZeneca AB, Sweden.

[0042] 2.2 Experimental grouping

[0043] The experiment was divided into 3 groups, namely normal group (n=3), sham operation group and ropivacaine group (n=3).

[0044] This experimental study complied with the standard requirements of the Animal Protection Committee, and the animals were anesthetized during the experiment to minimize the pain of the animals.

[0045] 2.3 Preoperative preparation

[0046] The motor nerve function of the mice in each group was scored before operation, and those with limb dysfunction were excluded. And Von-Frey method was used to measure the mechanical leg withdrawal threshold of mice, which was used for postoperative control.

[0047] 2.4 Mo...

Embodiment 3

[0054] 3.1 Experimental materials and animal groups

[0055]Two-month-old male C57 mice were selected, weighing 20-30 g, fed at room temperature (23±2)°C, light control for 12 hours (7:00am to 7:00pm), and free access to water. 1% ropivacaine hydrochloride was purchased from AstraZeneca AB, Sweden.

[0056] 3.2 Experimental Grouping

[0057] The experiment was divided into 3 groups, namely normal group (n=3), sham operation group and ropivacaine group (n=3).

[0058] This experimental study complied with the standard requirements of the Animal Protection Committee, and the animals were anesthetized during the experiment to minimize the pain of the animals.

[0059] 3.3 Preoperative preparation

[0060] The motor nerve function of the mice in each group was scored before operation, and those with limb dysfunction were excluded. And Von-Frey method was used to measure the mechanical leg withdrawal threshold of mice, which was used for postoperative control.

[0061] 3.4 Mod...

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Abstract

The invention discloses an establishment method of a sciatic nerve injury model, the establishment method comprises the following steps: selecting a 2-month-old mouse with the weight of 20-30g, after anesthesia, exposing a sciatic nerve trunk to a sciatic nerve bifurcation, injecting ropivacaine hydrochloride around the sciatic nerve, and after 24 hours, injecting ropivacaine hydrochloride around the sciatic nerve again; injecting 2-5ul / g of 1% ropivacaine hydrochloride each time. According to the invention, 1% ropivacaine hydrochloride is injected around the sciatic nerve to cause the sciatic nerve injury of the mouse, the clinical peripheral nerve block environment is simulated, and the model is the most ideal animal model for researching the neurotoxicity injury of local anesthetics. According to the invention, the sciatic nerve trunk is exposed to the sciatic nerve bifurcation, and the 1% ropivacaine hydrochloride is given under the direct vision, such that the sciatic nerve can be wrapped by the ropivacaine hydrochloride can be ensured, and the nerve injury caused by the peripheral nerve block due to the clinical long-time high concentration of the local anesthetic can be simulated;.

Description

technical field [0001] The invention relates to the research field of nerve injury diseases, in particular to a method for establishing a sciatic nerve injury model. Background technique [0002] Peripheral nerve injury often manifests as paresthesia, numbness, regression or hyperactivity, spasm, allodynia, electric shock-like pain and other symptoms. There are many situations that cause peripheral nerve injury. The existing research on peripheral nerve injury mainly focuses on the construction of injury models of isolated cells, or the construction of animal models through physical intervention. The isolated cell injury model can only understand the changes of the model subjects through analysis and testing methods; animal models are easier to observe the changes of the subjects, but because the physical intervention method requires additional fixtures or the need to directly destroy nerve cells, It has high construction difficulty. Contents of the invention [0003] Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D1/00
CPCA61D1/00
Inventor 文先杰江浩陈美欣刘晓鹏唐贵娟毋朝霞杨淑璇艾贵珍
Owner 佛山市第二人民医院
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