Application of 5-Pyridoxolactone in preparation of medicine for treating bombyx mori nuclear polyhedrosis virus (BmNPV)

A technology of karyotype polyhedron and silkworm, which is applied in the field of biomedicine and achieves the effect of good safety

Active Publication Date: 2021-07-16
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no effective drug report for the silkworm disease caused by the virus infection. Therefore, there is an urgent need for a method that can quickly and efficiently inhibit the infection of the silkworm baculovirus (BmNPV), so as to provide information on the silkworm karyotype for the research on the mechanism of silkworm resistance to BmNPV virus and the silkworm production. Laying the foundation for the prevention and treatment of polyhedrosis virus disease

Method used

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  • Application of 5-Pyridoxolactone in preparation of medicine for treating bombyx mori nuclear polyhedrosis virus (BmNPV)
  • Application of 5-Pyridoxolactone in preparation of medicine for treating bombyx mori nuclear polyhedrosis virus (BmNPV)
  • Application of 5-Pyridoxolactone in preparation of medicine for treating bombyx mori nuclear polyhedrosis virus (BmNPV)

Examples

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Effect test

Embodiment 1

[0019] Example 1, using 5-Pyridoxolactone in vitro to suppress silkworm nuclear polyhedron virus replication

[0020] Use 5-Pyridoxolactone in vitro to inhibit silkworm nuclear polyhedral virus replication, the specific steps are as follows:

[0021] A. Remove the frozen Bme cell frozen tube in liquid nitrogen, immediately placed in a 37 ° C water bath, shake from time to time, melting it within 1 minute; then BME cells are transferred to GRACE medium, mix well, then Centrifuge under 1200 rpm / minute; discarded, add 10% (v / v) fetal bovine serum grace insect cell medium, mix, then transfer cells into the culture flask, cultured at 27 ° C Light 12H in the box, dark dark 12h;

[0022] B. When the drug is detected to cytotoxicity, the cells growing to 95% are conducted and spread, cultivated overnight;

[0023] C. Settings without cells contain only medium blank groups, non-dosing control groups and additional 5-pyridoxolactone final concentrations of 0, 2.5, 5, 10, 20 μm, continue...

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Abstract

The invention discloses application of 5-Pyridoxolactone in preparation of a medicine for treating bombyx mori nuclear polyhedrosis virus (BmNPV). Micromolecule 5-Pyridoxolactone in an anti-BmNPV host cell is reasonably utilized to inhibit the replication of BmNPV in the cell, that is, after the cell is infected by the BmNPV, the 5-Pyridoxolactone is firstly added to inhibit the replication of the BmNPV, so that a new thought is provided for treating the BmNPV infection of the cell, and a foundation is laid for virus infection prevention and treatment in sericulture production.

Description

Technical field [0001] The present invention relates to the field of biopharmaceutical, and specific to 5-pyridoxolactone is used to prepare applications of drugs for the treatment of silkworm nuclear polyhedron viruses. Background technique [0002] Silk Word Nuclear Multicore Virus (BMNPV) belongs to the mainly infected infestor, especially insects. BMNPV infection is a silkworm disease that is harmful, difficult to prevent, and have no effective control measures. BMNPV virus rod virus is mainly divided into nuclear polyhedron viruses and particulate viruses by inclusions. The genomic size of the BMNPV virus is 128kB, which contains 136 genes. [0003] BMNPV viruses produce two different types of viruses in two developmental ages, one for inclusion body derivative (ODV), and the other is a sprouting virus (BV). Different BV viruses and ODV viruses, the BV virus has a sachet of the protein, while the ODV virus particles are wrapped in inclusions. The BV virus mainly infects cell...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4355A61P31/20
CPCA61K31/4355A61P31/20Y02A50/30
Inventor 夏庆友化晓婷
Owner SOUTHWEST UNIVERSITY
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