Penaees chinensis PcRab7 protein and WSSV envelope protein VP28 interaction blocker polypeptide
An envelope protein, shrimp technology, applied in the field of biomedicine to achieve the effect of prolonging the survival time
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[0017] The polypeptide (TAPLAITAIIAVFI) of the present invention was synthesized by a biological company.
[0018] The live Penaeus chinensis is cultured by the applicant's experimental base.
[0019] ① Virus extraction: Homogenate the virus-infected prawn tissue with 5 times the volume (v / v) of TNE (plus PMSF), and centrifuge the homogenate at 2500×g at 4°C for 10 minutes. Take the supernatant and centrifuge at 10000×g for 30min at 4°C. Pass through a 0.45 μm filter membrane. The supernatant was taken and centrifuged at 100000×g for 1h at 4°C. The extracted WSSV particles were suspended in sterile phosphate buffered saline (PBS);
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