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Penaees chinensis PcRab7 protein and WSSV envelope protein VP28 interaction blocker polypeptide

An envelope protein, shrimp technology, applied in the field of biomedicine to achieve the effect of prolonging the survival time

Active Publication Date: 2021-07-16
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although the precise mechanism of WSSV virus entry into the shrimp body is still unknown, related studies have shown that the virus enters host cells through the interaction of the virus envelope protein with the host cell receptors

Method used

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  • Penaees chinensis PcRab7 protein and WSSV envelope protein VP28 interaction blocker polypeptide
  • Penaees chinensis PcRab7 protein and WSSV envelope protein VP28 interaction blocker polypeptide

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Experimental program
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Effect test

Embodiment 1

[0017] The polypeptide (TAPLAITAIIAVFI) of the present invention was synthesized by a biological company.

[0018] The live Penaeus chinensis is cultured by the applicant's experimental base.

[0019] ① Virus extraction: Homogenate the virus-infected prawn tissue with 5 times the volume (v / v) of TNE (plus PMSF), and centrifuge the homogenate at 2500×g at 4°C for 10 minutes. Take the supernatant and centrifuge at 10000×g for 30min at 4°C. Pass through a 0.45 μm filter membrane. The supernatant was taken and centrifuged at 100000×g for 1h at 4°C. The extracted WSSV particles were suspended in sterile phosphate buffered saline (PBS);

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Abstract

The invention provides a blocking polypeptide. The blocking polypeptide can block the interaction between PcRab7 protein of Penaees chinensis and WSSV envelope protein VP28, and has an anti-WSSV effect. The amino acid sequence of the blocking polypeptide provided by the invention is TAPLAITAIIAVFI. The polypeptide provided by the invention can block the binding of PcRab7 to VP28, and can further influence the fusion of a virus envelope and a host cell membrane, so that the virus can not enter a host cell, and the anti-WSSV effect is realized. According to the invention, a Penaees chinensis infection experiment is adopted, and the experiment proves that the polypeptide can effectively prolong the survival time of Penaees chinensis infected with WSSV, and the polypeptide can be used as an effective anti-WSSV candidate drug, providing possible polypeptide drugs for prevention and treatment of WSSV in the future.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to blocking the interaction between the Chinese prawn protein PcRab7 and the white spot syndrome virus envelope protein VP28, and inhibiting the white spot syndrome virus from infecting the Chinese prawn. Background technique [0002] Shrimp white spot syndrome is a viral disease caused by White Spot Syndrome Virus (WSSV), which endangers the global shrimp farming industry. The virus has a wide range of hosts and strong toxicity. It has a 90%-100% lethality rate within 3 to 7 days after infecting prawns, seriously endangering the prawn farming industry. Therefore, screening for efficient antiviral drugs is of great significance. [0003] At present, the main countermeasures and control measures of WSSV prevention and control in the actual breeding process have played a role in the prevention and control of the epidemic of WSSV to a certain extent, but there is still no effectiv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08A61K38/10A61P31/20
CPCC07K7/08A61P31/20A61K38/00
Inventor 纪晓峰郑媛盛军
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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