3 results about "Entry into host cell" patented technology

This invention provides a chimeric adenovirus vector Ad5F35 and a recombinant CCR5-32 mutant gene, its construction method, and its application, belonging to the field of gene therapy technology. The construction method provided by this invention includes the following steps: (1) inserting the CCR5Δ32 mutant gene into the multiple cloning site of a shuttle vector to obtain a shuttle plasmid; (2) co-transfecting cells with the shuttle plasmid and the Ad5F35 adenovirus backbone plasmid to generate an Ad5F35 chimeric recombinant adenovirus capable of expressing the CCR5Δ32 gene. By inducing the inability of the CCR5 protein on the host cell to be normally expressed on the cell membrane surface, the binding of HIV-1 gp 120 to the CCR5Δ32 mutant gene is effectively prevented, thus preventing the HIV-1 virus from entering the host cell for replication, thereby achieving the goal of treating AIDS.

The application provides application of PLX8394 in preparation of an anti-ZIKV drug, a structural formula of the PLX8394 is shown as formula 1, the application firstly finds that the PLX8394 can be used for treating infectious diseases caused by ZIKV, and experiments show that the PLX8394 has extremely strong antiviral activity on ZIKV, can significantly inhibit replication of ZIKV on host cells A549, improve cell survival rate, can inhibit the process of virus entering the host cells A549, and has extremely strong antiviral effect at a cell level, and has obvious anti-ZIKV effect.

The application discloses an antiviral composite particle and a screening method and application thereof. The application utilizes a polymer reversible hydrogen bond network with hydrophilicity and hydrophobicity, precisely regulates a coordination mode of a metal oligomer, balances material dispersibility and virus capturing capacity by adjusting a charge distribution of a polymer main chain, and controls a material surface to form an electrostatic barrier through a surface potential, so that the material selectively adsorbs virus particles without entering host cells, and cell toxicity is avoided. In addition, through the synergistic design of controllable assembly of the metal oligomer and polymer interface engineering, the application breaks through the dilemma of the trade-off between "activity-toxicity" of traditional metal-based materials, and provides strong support for the development of broad-spectrum, safe antiviral drugs, coatings, protective equipment and the like.