Carrier for gene transmission and preparation method thereof and application
A gene delivery and carrier technology, applied in the field of non-viral carrier-gelatin-siloxane nano-hybrid materials and its preparation, can solve the problems of non-viral nanoparticle gene carrier instability and low transfection rate, and achieve non-viral Immunogenicity, no side effects, good biocompatibility
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Embodiment 1
[0033] 0.2 g of gelatin was dissolved in 20 mL of acetic acid solution with pH 3 and stirred at 50° C. for 30 min. Then 0.2 g of 3-(2,3-glycidoxy)propyltrimethoxysilane was added to the above 1% gelatin solution. After the reaction solution was stirred at 30° C. for 6 hours, ammonia water was added to adjust the pH of the solution to 9, and the stirring reaction was continued for 12 hours to obtain a milky white suspension. The milky white suspension was subjected to high-speed centrifugation (20,000 rpm, 15 min, 30° C.) to obtain a white precipitate. The white precipitate was washed twice with distilled water to obtain the following figure 2 (a) Gelatin-siloxane nanohybrid material with a size of approximately 150 nm shown. Characterized by infrared spectrum, such as figure 1 shown, 1650cm -1 is the stretching vibration absorption peak of carbonyl-C=O, 1541cm -1 is amino-NH 2 Bending vibrations, both derived from gelatin molecules, 1030 and 1140 cm -1 is the stretchin...
Embodiment 2
[0035] 0.02 g of gelatin was dissolved in 20 mL of hydrochloric acid solution with pH=3, and stirred at 50° C. for 30 min. Then 0.2 g of 3-(2,3-glycidoxy)propyltrimethoxysilane was added to the above 0.1% gelatin solution. After the reaction solution was stirred at 50° C. for 30 min, 3-(trimethoxysilane)propylamine was added to adjust the pH of the solution to 9, and the reaction was continued to be stirred for 12 hours to obtain a milky white suspension. The milky white suspension was subjected to high-speed centrifugation (20,000 rpm, 15 min, 30° C.) to obtain a white precipitate. The white precipitate was washed twice with distilled water to obtain the following figure 2 (b) Gelatin-siloxane nanohybrid material with a size of about 600 nm shown.
Embodiment 3
[0037]0.5 g of gelatin was dissolved in 20 mL of hydrochloric acid solution with pH=3, and stirred at 50° C. for 30 min. Then 0.25 g of 3-(2,3-glycidoxy)propyltrimethoxysilane was added to the above 2.5% gelatin solution. After the reaction solution was stirred at 50° C. for 30 minutes, 3-(trimethoxysilane)propylamine was added to adjust the pH to 10.0, and the stirring reaction was continued for 12 hours to obtain a milky white suspension. The milky white suspension was subjected to high-speed centrifugation (20,000 rpm, 15 min, 30° C.) to obtain a white precipitate. The white precipitate was washed twice with distilled water to obtain the following figure 2 (c) Gelatin-siloxane nanohybrid material with a size of 20 nm shown.
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