Application of Vina-ginsenoside R18 in preparation of anti-dengue virus pharmaceutical preparation
A pharmaceutical preparation, dengue virus technology, applied in the field of anti-virus, to achieve the effect of inhibiting replication, strong anti-dengue virus activity, and good medicinal prospects
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Embodiment 1
[0036] Example 1 Toxicity of Vina-ginsenoside R18 to BHK-21 cells
[0037] Test method BHK-21 cells in the logarithmic growth phase were taken in 1×10 5 Cells / mL were inoculated in 96-well plates, 100 μL per well, and the control group and drug groups with different concentrations were set up. The cytotoxicity was detected by MTT method 4 days later.
[0038] figure 1 The test results of Vina-ginsenoside R18 showed that the half inhibitory concentration of Vina-ginsenoside R18 on BHK-21 cells was 54.11 μM, and it was basically non-toxic to cells at a concentration of 25 μM.
Embodiment 2
[0039] Example 2 Vina-ginsenoside R18 blocks the adsorption of type 2 dengue virus to host cells
[0040] Test method BHK-21 cells in the logarithmic growth phase were taken in 1×10 5 Cells / mL were seeded in a 96-well plate with 100 μL per well. The model group was infected with 200PFU of DENV-2, and the drug group was given 200PFU of DENV-2 and vina-ginsenoside R18 (6.25, 12.5, 25μM) at the same time. After incubation at 4°C for 1 hour, the virus and drug solution were discarded, and 2% maintenance solution was added. , placed in 37 ℃, 5% (v / v) CO 2 continue to grow in the incubator. After 4 days, the blocking effect of the drug on the adsorption of type 2 dengue virus to the host cells was determined by observing the cell lesions and death.
[0041] figure 2 The experimental results showed that Vina-ginsenoside R18 blocked the adsorption of dengue virus type 2 to host cells in a dose-dependent manner.
Embodiment 3
[0042] Example 3 Vina-ginsenoside R18 blocks type 2 dengue virus from entering host cells
[0043] Test method BHK-21 cells in the logarithmic growth phase were taken in 1×10 5 Cells / mL were seeded in a 96-well plate with 100 μL per well. The model group and the drug group were respectively infected with 200PFU of DENV-2, cultured at 4°C for 1 hour, discarded the virus, and the drug group was given vina-ginsenoside R18 (6.25, 12.5, 25μM), placed in 37°C, 5% (v / v )CO 2 Incubate for 1 h in an incubator. Afterwards, the drug solution was discarded, 2% maintenance solution was added, and the culture was continued in an incubator at 37°C. After 4 days, the blocking effect of the drug on the entry of type 2 dengue virus into the host cells was determined by observing the lesions and death of the cells.
[0044] image 3 The test results show that Vina-ginsenoside R18 can effectively block type 2 dengue virus from entering host cells.
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