Portable staphylococcus detection method and device based on micro-fluidic paper chip

Abstract

The invention discloses a portable staphylococcus detection method and device based on a micro-fluidic paper chip and belongs to the field of biomedical detection. The method comprises the following steps of: controlling a sample solution to flow into a culture and color development unit, and then enabling a staphylococcus aureus enterotoxin A specific antibody solution to flow into the culture and color development unit; flushing an unattached redundant primary antibody solution in the culture and color development unit with a PBS buffer solution; enabling a peroxidase anti-enterotoxin A specific antibody solution to flow into the culture and color development unit; flushing an unattached redundant secondary antibody solution in the culture and color development unit with the PBS buffer solution again; enabling a peroxide substrate OPD solution to flow into the culture and color development unit; and enabling an enzyme on the surface of the secondary antibody combined with the surface of staphylococcus aureus to decompose the substrate to generate a colored reaction product, and enabling a stop solution to destroy peroxidase after completing the reaction,so as to make the enzyme-linked substrate not to be decomposed any more. According to the method and device of the invention, a microfluidic paper-based chip technology is combined with an ELISA detection technology, and a concentration value can be obtained by taking a picture of a color developing area by a mobile phone and then carrying out HSV analysis.

Description

technical field [0001] The invention belongs to the field of biomedical detection, and specifically relates to a staphylococcus aureus detection device and method based on enzyme-linked immunoassay technology (ELISA) and a composite microfluidic paper-based chip, which can be used to rapidly detect the concentration of staphylococcus aureus in any solution. Handheld detection. Background technique [0002] Staphylococcus aureus (Staphylococcus aureus) is the main pathogenic bacterium that induces daily diseases in humans and animals. It is a serious threat to food safety. When the concentration exceeds the standard, it can cause food poisoning. It has always been the key object of the supervision of biological pollutants in food. Staphylococcus aureus can contaminate almost all food, especially the threat to meat and its products is particularly serious, known as "flesh-loving bacteria"; its risk of harm to food quality and safety is mainly caused by a large number of invas...

AI Technical Summary

Problems solved by technology

[0004] Using the traditional medium colony observation method, there are many defects such as large environmental influence factors, low concentration measurement range, easy contamination in the detection process, and low detection accuracy. At the same time, improper handling of the medium after use is very prone to serious biological contamination. as a result of

For example, the Chinese patent number is ZL200520120355.0, and the test strips used in the name "Testing strips for pathogenic microorganisms in food" are expensive and need to be used in conjunction with peripheral equipment such as constant temperature incubators. The method of manually counting colonies is used to obtain the concentration , not suitable for quick and easy home testing

However, the laboratory ELISA measurement method with extremely high precision has a cumbersome measurement process, expensive instruments, and requires a professional sterile environment, which obviously does not meet the needs of people's daily home use.

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