Application of polyprezinc in the preparation of drugs for treating castration-resistant prostate cancer
A technology of castration resistance and polyprezinc, which is applied in the field of biomedicine, can solve the problems of drug resistance in CRPC and achieve the effect of shortening the time for clinical transformation
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Embodiment 1EP
[0029] Embodiment 1EPI, Enza are used for the process that prostate cancer LNCaP cell produces DTP
[0030] EPI, Enza-resistant prostate cancer L-DTP cell lines L-DTP-EPI, L-DTP-Enza have recoverable properties.
[0031] 1. Experimental method:
[0032] LNCaP cells 1x10 6 Each species was planted in a 10cm cell culture dish, and after adhering to the wall on the second day, EPI and Enza were added for 9 days, during which the fresh medium containing the drug was changed every three days; after 9 days, the drug was withdrawn, and the medium was replaced with a fresh medium and placed in the incubator Cells were normally cultured in medium, passaged on day 6, day 12, and day 17, and the cell morphology of DTP (day 9), R5 (day 5 of drug withdrawal), R10, and R20 were photographed under an inverted microscope. After producing DTP and cloned DTP cells, the cells were digested and counted, and the percentage of DTP cells to the total L-parent cells was calculated. L-parent cells ...
Embodiment 2D
[0037] Example 2 Characteristic establishment of AR-related gene expression changes in DTP and Recovery Cell cells
[0038] The cell morphology of DTP cells recovered after 3 passages of drug withdrawal, and the changes in the expression of AR-related genes in the cells could also be restored at this time.
[0039] 1. Experimental method:
[0040] LNCaP cells were placed in a 10cm dish, after overnight adherence to the wall, EPI and Enza were added to treat the cells for 9 days, and then the cells were collected. During this period, the fresh medium added with drugs was replaced every 3 days, and the remaining DTP cells were collected 20 days after drug dispensing. Set LNCaP cells treated with DMSO for 9 days as the NC control group, and treated with EPI and Enza for 9 days to produce drug-resistant cells of L-DTP-EPI and L-DTP-Enza as the treatment group, and the cells of this treatment group after withdrawal of the drug for 20 days were In the recovery group, these three gr...
Embodiment 4
[0049] Example 4 The effect of Enza and Pola combined drug regimen on the C-MYC overexpression prostate cancer mouse model after the continuous drug Enza produces resistance
[0050] Further, in the prostate cancer mouse model, the effect of combined administration of Enza and Pola on relapsed mice after chemical castration (ie continuous administration of Enza) was illustrated.
[0051] 1. Experimental method
[0052] Construct a spontaneous prostate cancer mouse model with C-MYC (Hi-Myc) overexpression. At 4 months, the mice developed mPIN / Cancer transition. At this time, the mice were randomly divided into NC control group (gavage solution), Enza drug After that, Enza was administrated once every three days, and Enza was 10mg / Kg for a total of 30 days. Afterwards, some mice were neck-broken and their prostate cancer was taken to take pictures and weighed. It was found that Enza could significantly alleviate the symptoms. The NC control group was reduced by half, and the re...
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