Method, device and equipment for evaluating immunity level and storage medium

Abstract

The invention provides a method, device and equipment for evaluating the immunity level and a storage medium. The method comprises the following steps: (1) collecting sequencing data of a B cell antigen receptor variable region and / or a T cell antigen receptor variable region of a lymphocyte sample, comparing the sequencing data with data in a public database, and calculating a D value; (2) distinguishing healthy samples and sub-healthy samples according to the distribution diagram of the D values, fitting the D values of the healthy samples by adopting multiple Gaussian distribution, and determining a standard D value; (3) fitting the D values of the sub-health sample and the disease sample by adopting a Gaussian distribution function to obtain a Gaussian distribution function A and a Gaussian distribution function B; (4) evaluating the immunity level of the unknown sample according to the standard D value, the Gaussian distribution function A and the Gaussian distribution function B. According to the method, the sequencing result is analyzed and subjected to multiple Gaussian distribution fitting, and the effect of accurately, comprehensively and efficiently evaluating the immunity of the sample is achieved.

Description

technical field [0001] The invention belongs to the technical field of immunization, and relates to a method, device, equipment and storage medium for evaluating immunity level. Background technique [0002] At present, the clinically applied immune function analysis methods mainly include immune five items, blood routine and lymphocyte subset analysis. Among them, the five items of immunity use one-way immunodiffusion method, enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (RIA), immunofixation electrophoresis or immunoturbidimetry to detect IgG, IgA, IgM, complement C3 and C4 in blood Blood routine uses cell counting to analyze the number of white blood cells in peripheral blood to determine whether there is an inflammatory response in the body; lymphocyte subset analysis uses flow cytometry or PCR technology to analyze the number of different white blood cell subsets in peripheral blood and relative proportions. [0003] However, these methods have drawbacks...

AI Technical Summary

Problems solved by technology

However, its accuracy needs to be further improved

[0005] Therefore, most of the existing immune detection methods can only be roughly evaluated, lacking comprehensiveness, sensitivity and accuracy, and it is necessary to establish a new method for immunity evaluation

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