Preparation method and application of flavonoid glycoside monomer isovitexin of Hydrocotyle sibthorpioides

A technology of cilantro flavonoid glycosides and isovitexin, which is applied in the field of preparation of isovitexin, a flavonoid glycoside monomer of coriander, can solve the problems that has not yet been found , to achieve the effect of clear and efficient chemical structure

Pending Publication Date: 2021-08-13
GUANGXI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the application of isovitexin, a flavonoid glycos...

Method used

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  • Preparation method and application of flavonoid glycoside monomer isovitexin of Hydrocotyle sibthorpioides
  • Preparation method and application of flavonoid glycoside monomer isovitexin of Hydrocotyle sibthorpioides
  • Preparation method and application of flavonoid glycoside monomer isovitexin of Hydrocotyle sibthorpioides

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preparation example Construction

[0028] In a specific embodiment, it firstly relates to a preparation method of isovitexin, a flavonoid glycoside monomer of coriander, the steps are as follows:

[0029] 1) Add coriander cypress into ethanol aqueous solution, first use ultrasonic wave to extract, then continue to leach in ethanol aqueous solution, filter, filter residue is repeatedly leached several times with ethanol aqueous solution, combine the filtrate, concentrate under reduced pressure until the ethanol is volatilized, and obtain ethanol crude extract;

[0030] 2) extracting the crude ethanol extract with petroleum ether, chloroform, and ethyl acetate in sequence, collecting the ethyl acetate extract, concentrating and drying to obtain the ethyl acetate extract;

[0031] 3) Separating the ethyl acetate extract by silica gel column chromatography, first using ethyl acetate-methanol-formic acid as the mobile phase for gradient elution, collecting the eluent, and then recrystallizing with methanol to obtain...

Embodiment 1

[0033] The preparation of embodiment 1 isovitexin

[0034] After fully pulverizing 10 kg of dried coriander, add 100 L of 75% ethanol aqueous solution, stir evenly, and use ultrasonic wave to extract.

[0035] The isovitexin that has been pretreated by ultrasound is extracted in an aqueous ethanol solution, filtered, and the obtained filter residue is repeatedly extracted 5 times. The temperature of each extraction is 25 ° C, and the time of each extraction is 25 hours. The filtrates were combined, and the filtrate was concentrated under pressure until the ethanol was evaporated, and finally the ethanol crude extract was obtained;

[0036] The crude ethanol extract was extracted with petroleum ether, chloroform, and ethyl acetate in sequence, and the dosages of the three were 1 / 2, 2 / 3, and 3 / 2 of the volume of the crude ethanol extract, respectively, extracted 3 times each, and ethyl acetate was collected. The ester extract was concentrated and dried to obtain an ethyl acetat...

Embodiment 2

[0038] The preparation of embodiment 2 isovitexin

[0039] After fully pulverizing 10kg of dried coriander, add 100L of 70% ethanol aqueous solution, stir evenly, and use ultrasonic wave to extract.

[0040] The isovitexin that has been pretreated by ultrasound is extracted in an aqueous ethanol solution, filtered, and the obtained filter residue is repeatedly leached 7 times. The temperature of each leaching is 20 ° C, and the time of each leaching is 20h. The filtrates were combined, and the filtrate was concentrated under pressure until the ethanol was evaporated, and finally the ethanol crude extract was obtained;

[0041] The crude ethanol extract was extracted with petroleum ether, chloroform, and ethyl acetate in sequence, and the dosages of the three were respectively 1 / 4, 4 / 3, and 5 / 3 of the volume of the crude ethanol extract, and each was extracted twice, and ethyl acetate was collected. The ester extract was concentrated and dried to obtain an ethyl acetate extrac...

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Abstract

The invention discloses a preparation method and application of a flavonoid glycoside monomer isovitexin of Hydrocotyle sibthorpioides. The method comprises the following steps: 1) adding Hydrocotyle sibthorpioides into an ethanol solution, performing ultrasonic extraction, then performing digestion with the ethanol solution multiple times, and performing vacuum concentration until ethanol is completely volatilized to obtain a crude ethanol extract; 2) sequentially extracting the crude ethanol extract with petroleum ether, chloroform and ethyl acetate, and successively conducting concentrating and drying to obtain an ethyl acetate extract; and 3) carrying out silica gel column chromatographic separation on the ethyl acetate extract, carrying out gradient elution with ethyl acetate, methanol and formic acid successively, collecting eluate, and performing recrystallizing with methanol to obtain a product. According to the application, the isovitexin is used for preparing a medicine used for preventing and treating the non-alcoholic fatty liver disease, and the protection mechanism of the isovitexin is inflammation prevention, anti-oxidative stress, lipid peroxidation and down-regulation of endoplasmic reticulum stress related protein expression. The invention provides a novel compound with a clear structure and high efficiency for the treatment of the non-alcoholic fatty liver disease, and develops a new medication approach.

Description

technical field [0001] The invention relates to the technical field of natural extracts, in particular to a preparation method and application of isovitexin, a flavonoid glycoside monomer of coriander coriander. Background technique [0002] Hydrocotyle sibthorpioides Lam. is a plant of the genus Hydrocotyle sibthorpioides Lam. The whole herb is used as medicine, which has the functions of clearing away heat and dampness, detoxifying and reducing swelling. Isovitexin is one of the important medicinal ingredients contained in coriander. In the prior art, for example, patent document CN106008483A discloses a method for extracting isovitexin from mung beans and the application of isovitexin in the preparation of cardioprotective drugs. Another example is the patent document CN109232548B, which discloses a method for extracting high-purity vitexin and isovitexin from sandalwood leaves. Another example is the patent document CN105085498B, which discloses a method for extracting...

Claims

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Application Information

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IPC IPC(8): C07D407/04A61P1/16A61P29/00A61P39/06A61K31/352
CPCC07D407/04A61P1/16A61P29/00A61P39/06A61K31/352
Inventor 黄瑀莘韦锦斌林兴陈思韵庞丽君冯钟文朱武嫦苏宏梅
Owner GUANGXI MEDICAL UNIVERSITY
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