Streptococcus thermophilus STN26, bacterial powder and application of streptococcus thermophilus STN26 in uric acid reducing products
A Streptococcus thermophilus, STN26 technology, applied in the field of microorganisms, can solve the problems of poor microbial viability, poor stability of curative effect, influence on microbial colonization, etc., and achieve the effects of convenient taking, good activity, good adaptability and customization ability.
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Embodiment 1
[0028] Example 1 Isolation and Identification of Bacterial Strains
[0029] 1. Strain screening and purification
[0030] (1) Sample preparation:
[0031] ①Put sterilized saline (0.85%) into a sterile triangular flask, then add 1ml of naturally fermented yoghurt from a herdsman's home in Yili, Xinjiang, to it, shake, and set aside;
[0032] ② Carry out 10 times gradient dilution to the solution of step ①, respectively 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 ,stand-by;
[0033] (2) Prepare medium:
[0034] The composition of the MRS plate medium is as follows:
[0035] Peptone 10g, beef powder 5g, sodium acetate trihydrate 5g, dipotassium hydrogen phosphate heptahydrate 2g, Tween 80 1mL, manganese sulfate tetrahydrate 0.05g, triammonium citrate 2g, glucose 20g, magnesium sulfate heptahydrate 0.2g, agar 15g, distilled water 1000mL; mix the above raw materials, adjust the natural pH, stir the bacterial liquid, and sterilize at 121°C, 0.1MPa for 20min; ;
[00...
Embodiment 2
[0048] Embodiment 2 preparation product
[0049] (1) Preparation of MRS liquid medium:
[0050] The composition of MRS liquid medium is peptone 10g, beef powder 5g, sodium acetate trihydrate 5g, dipotassium hydrogen phosphate heptahydrate 2g, Tween 80 1mL, manganese sulfate tetrahydrate 0.05g, triammonium citrate 2g, glucose 20g, seven Magnesium sulfate water 0.2g, distilled water 1000mL; after mixing the above raw materials, adjust the pH to 6.8, stir the bacteria solution, and sterilize at 121°C and 0.1MPa for 20min.
[0051] (2) Preparation of bacteria powder:
[0052] The Streptococcus thermophilus STN26 preserved in Example 1 was inoculated on the MRS plate medium, activated and cultured at 37°C for 48h, then inoculated in the MRS liquid medium according to the inoculum size of 1%, and cultivated at 37°C for 24h , to obtain the bacterial liquid;
[0053] After the bacterial liquid is centrifuged, the bacterial cells are collected, washed with sterile physiological sali...
Embodiment 3
[0056] Example 3 Streptococcus thermophilus STN26 passed the simulated gastrointestinal digestive juice experiment
[0057] 1. Preparation and operation of simulated gastric juice
[0058] (1) The simulated gastric juice was prepared according to the following method: dilute with 9.5% hydrochloric acid and distilled water to pH 1.5, add 1.0 g of pepsin per 100 mL, mix well, filter with a 0.22 μm sterile filter membrane, and prepare and use immediately.
[0059] (2) Experimental process:
[0060] ①Weigh 1g of the product of specification B, as a sample, for use;
[0061] ②Take 9mL of simulated gastric juice from step (1) in a test tube, preheat it to 37°C, then put the sample in step ① into the test tube, and place it at 37°C and 80r / min for 1h, 2h, 3h and 4h respectively. Each treatment was repeated 3 times;
[0062] ③ Detection: Gradiently dilute the treated sample solution, use the pouring method to count viable bacteria, and then calculate the survival rate. The results ...
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