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Probe, detection test strip and application thereof

A technology for testing test strips and test strips, applied in biological tests, measuring devices, material inspection products, etc., can solve the problems of cumbersome sample pretreatment steps and time-consuming processes, hindering applications, expensive instruments, etc., and achieve excellent stability. , high specificity and high sensitivity

Active Publication Date: 2021-08-13
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these conventional methods can provide highly sensitive detection and obtain accurate results, they require expensive instruments, professional operators, cumbersome sample pretreatment steps and time-consuming processes, which hinder their application in on-site and rapid detection. application
For more sensitive detection of albuterol, especially rapid on-site detection, it is still a great technical challenge to realize an easy-to-use rapid, convenient and inexpensive method

Method used

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  • Probe, detection test strip and application thereof
  • Probe, detection test strip and application thereof
  • Probe, detection test strip and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1: Optimization of conditions for polydopamine-modified iridium oxide nanoparticles

[0058] combine figure 1 , the present embodiment provides the preparation method of iridium oxide nanoparticles and optimizes the amount of dopamine hydrochloride solution added, including the following steps:

[0059] (1) Preparation of iridium oxide nanoparticles (IrO 2 NPs): mix 50mg trisodium citrate and 30mg K 2 IrCl 6 Mix with 50 mL of ultrapure water, adjust the pH of the solution to 7.5, and reflux under boiling. When the color of the solution changed from brown to steel gray, the solution was continued to be stirred and heated for 30 minutes. After the solution was cooled to room temperature, the pH was readjusted to 7.5, and then the solution was heated for 30 minutes until the pH was stable, and the color of the final solution turned dark blue to obtain IrO 2 NPs solution.

[0060] (2) Optimizing the amount of dopamine hydrochloride solution added: 15 μL o...

Embodiment 2

[0065] Embodiment 2: the preparation of the probe of the test strip of rapid detection albuterol

[0066] According to the above-mentioned technical scheme, this example provides the probe and its preparation method, including using polydopamine-modified iridium oxide nanoparticles as the signal carrier, and then adding salbutamol monoclonal antibody for adsorption. Include the following steps:

[0067] (1) Preparation of polydopamine-modified iridium oxide nanoparticles (IrO 2 @PDA): Add 15 μL of hydrogen peroxide solution (3%, v:v) into the iridium oxide nanoparticle solution (1 mL) and mix and stir for 10 minutes, then add 10 μL of dopamine hydrochloride solution (10 mg / mL), and stir overnight. Finally, the prepared IrO 2 @PDA was washed twice with deionized water and resuspended in ultrapure water, and stored in a 4°C refrigerator.

[0068] (2) Preparation of probes: add albuterol monoclonal antibody to polydopamine-modified iridium oxide nanoparticle solution in step (...

Embodiment 3

[0074] Embodiment 3: Preparation of the immunochromatographic test strip for rapid detection of albuterol

[0075] combine Figure 4 , the present embodiment provides the high-sensitivity immunochromatographic test strip of fast detection salbutamol, comprises nitrocellulose membrane, sample pad, conjugation pad and absorbent pad and backing plate, is attached with nitrocellulose membrane on the backing plate, and nitrocellulose One end of the plain membrane is covered with a water-absorbing pad, and the other end of the nitrocellulose membrane is covered with a sample pad and a binding pad in turn. The non-covered surface of the nitrocellulose membrane is provided with a detection line and a control line along the horizontal direction, and the binding pad and the sample pad are separated by blocking solution. closed processing.

[0076] The preparation method of the nitrocellulose membrane comprises: 1 mg / mL salbutamol-bovine serum albumin conjugate is coated on the detectio...

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Abstract

The invention discloses a probe, a detection test strip and application thereof. The probe comprises a monoclonal antibody and a signal carrier, wherein the signal carrier is polydopamine modified iridium oxide nanoparticles. According to the invention, the probe is constructed by using the polydopamine modified iridium oxide nanoparticles in immunodetection for the first time, high-sensitivity target detection can be realized, the detection limit is as low as 0.002 ng / mL, and compared with the sensitivity of a pure iridium oxide nanoparticle test strip and the sensitivity of a traditional gold-labeled test strip, the sensitivity is respectively improved by 24 times and 180 times. In addition, the probe has a good application prospect, and can be used as a general detection method for detecting various harmful micromolecule analytes in food. Meanwhile, the probe can be successfully applied to salbutamol detection in pork, pork liver and beef samples.

Description

technical field [0001] The invention belongs to the field of biological detection, and specifically relates to a method of using polydopamine-modified iridium oxide nanoparticles as a signal carrier, preparing a probe by labeling an antibody, a test strip using the probe, and its rapid detection application. Background technique [0002] In recent years, immunochromatographic test strips have received widespread attention due to their rapidity, high sensitivity, good specificity, and low cost, and have become an important means of on-site rapid detection, mainly for monitoring low-concentration analytes, Examples include viruses, cancer biomarkers, bacteria and toxins, etc. Gold nanoparticles (AuNPs), with unparalleled biocompatibility and unique optical properties, are commonly used signaling labels for the detection of SAL. However, the poor stability and low sensitivity of AuNP-based immunochromatographic test strips seriously hinder its further application. Fortunately...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/558G01N33/58G01N33/53G01N33/531G01N33/532
CPCG01N33/577G01N33/558G01N33/587G01N33/5308G01N33/531G01N33/532Y02A50/30
Inventor 王丽赵爽补彤白菲儿
Owner NORTHWEST A & F UNIV
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