A method for separating and extracting fillers between rice starch grains
An extraction method and technology of starch granules, which are applied in the field of separation and extraction of fillers between rice starch granules, and can solve problems such as ambiguity
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Embodiment 1
[0034] 1) Select the glutinous rice variety Dalinuo, weigh 100±0.1g of polished rice flour passed through a 100-mesh sieve into a 1000mL beaker, add 400mL 0.5mM AgNO 3 Solution, soaked at 4°C for 72h, wrapped in tin foil to avoid light to prevent AgNO 3 Breakdown to inhibit endogenous starch hydrolysis starch intergranular fillers. Stir 3-5 times during soaking;
[0035] 2) The polished rice flour suspension obtained in step 1) is placed on a magnetic stirrer and stirred at a speed of 300 rpm for 4-6 hours under the condition of avoiding light;
[0036] 3) Mix the suspension obtained in step 2) with 4 times the volume of glycerin with a purity ≥ 99.5%, and gently invert up and down and mix repeatedly;
[0037] 4) Put the mixture obtained in step 3) into a centrifuge bottle in a floor centrifuge, set the speed at 1200g / min, and centrifuge for 10min;
[0038] 5) Collect the precipitate and upper liquid obtained in step 4) respectively;
[0039] 6) Wash the precipitate obtain...
Embodiment 2
[0043] 1) Choose the japonica rice variety Chunjiang 151, weigh 100±0.1g of the polished rice flour of Chunjiang 151 through a 100-mesh sieve into a 1000mL beaker, add 400mL 0.5mM AgNO 3 Solution, soaked at 4°C for 72h, wrapped in tin foil to avoid light to prevent AgNO 3 Breakdown to inhibit endogenous starch hydrolysis starch intergranular fillers. Stir 3-5 times during soaking;
[0044] 2) The polished rice flour suspension obtained in step 1) is placed on a magnetic stirrer and stirred at a speed of 300 rpm for 4-6 hours under the condition of avoiding light;
[0045] 3) Mix the suspension obtained in step 2) with 4 times the volume of glycerin with a purity ≥ 99.5%, and gently invert up and down and mix repeatedly;
[0046] 4) Put the mixture obtained in step 3) into a centrifuge bottle in a floor centrifuge, set the speed at 1500g / min, and centrifuge for 10min;
[0047] 5) Collect the precipitate and upper liquid obtained in step 4) respectively;
[0048] 6) Wash the...
Embodiment 3
[0052] 1) Select Zhongjiazao 17, an indica rice variety, weigh 100±0.1g of polished rice flour passed through a 100-mesh sieve into a 1000mL beaker, add 400mL 0.5mM AgNO 3 Solution, soaked at 4°C for 72h, wrapped in tin foil to avoid light to prevent AgNO 3 Breakdown to inhibit endogenous starch hydrolysis starch intergranular fillers. Stir 3-5 times during soaking;
[0053] 2) The polished rice flour suspension obtained in step 1) is placed on a magnetic stirrer and stirred at a speed of 300 rpm for 4-6 hours under the condition of avoiding light;
[0054] 3) Mix the suspension obtained in step 2) with 4 times the volume of glycerin with a purity ≥ 99.5%, and gently invert up and down and mix repeatedly;
[0055] 4) Put the mixture obtained in step 3) into a centrifuge bottle in a floor centrifuge, set the speed at 2000g / min, and centrifuge for 10min;
[0056] 5) Collect the precipitate and upper liquid obtained in step 4) respectively;
[0057] 6) Wash the precipitate ob...
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