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A method for separating and extracting fillers between rice starch grains

An extraction method and technology of starch granules, which are applied in the field of separation and extraction of fillers between rice starch granules, and can solve problems such as ambiguity

Active Publication Date: 2022-04-15
CHINA NAT RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Since previous studies on starch intergranular fillers have not been reported, it is not yet clear what effect these detritus fillers have on rice quality and whether they function as hydrothermal media in the cooking and gelatinization process.

Method used

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  • A method for separating and extracting fillers between rice starch grains
  • A method for separating and extracting fillers between rice starch grains
  • A method for separating and extracting fillers between rice starch grains

Examples

Experimental program
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Effect test

Embodiment 1

[0034] 1) Select the glutinous rice variety Dalinuo, weigh 100±0.1g of polished rice flour passed through a 100-mesh sieve into a 1000mL beaker, add 400mL 0.5mM AgNO 3 Solution, soaked at 4°C for 72h, wrapped in tin foil to avoid light to prevent AgNO 3 Breakdown to inhibit endogenous starch hydrolysis starch intergranular fillers. Stir 3-5 times during soaking;

[0035] 2) The polished rice flour suspension obtained in step 1) is placed on a magnetic stirrer and stirred at a speed of 300 rpm for 4-6 hours under the condition of avoiding light;

[0036] 3) Mix the suspension obtained in step 2) with 4 times the volume of glycerin with a purity ≥ 99.5%, and gently invert up and down and mix repeatedly;

[0037] 4) Put the mixture obtained in step 3) into a centrifuge bottle in a floor centrifuge, set the speed at 1200g / min, and centrifuge for 10min;

[0038] 5) Collect the precipitate and upper liquid obtained in step 4) respectively;

[0039] 6) Wash the precipitate obtain...

Embodiment 2

[0043] 1) Choose the japonica rice variety Chunjiang 151, weigh 100±0.1g of the polished rice flour of Chunjiang 151 through a 100-mesh sieve into a 1000mL beaker, add 400mL 0.5mM AgNO 3 Solution, soaked at 4°C for 72h, wrapped in tin foil to avoid light to prevent AgNO 3 Breakdown to inhibit endogenous starch hydrolysis starch intergranular fillers. Stir 3-5 times during soaking;

[0044] 2) The polished rice flour suspension obtained in step 1) is placed on a magnetic stirrer and stirred at a speed of 300 rpm for 4-6 hours under the condition of avoiding light;

[0045] 3) Mix the suspension obtained in step 2) with 4 times the volume of glycerin with a purity ≥ 99.5%, and gently invert up and down and mix repeatedly;

[0046] 4) Put the mixture obtained in step 3) into a centrifuge bottle in a floor centrifuge, set the speed at 1500g / min, and centrifuge for 10min;

[0047] 5) Collect the precipitate and upper liquid obtained in step 4) respectively;

[0048] 6) Wash the...

Embodiment 3

[0052] 1) Select Zhongjiazao 17, an indica rice variety, weigh 100±0.1g of polished rice flour passed through a 100-mesh sieve into a 1000mL beaker, add 400mL 0.5mM AgNO 3 Solution, soaked at 4°C for 72h, wrapped in tin foil to avoid light to prevent AgNO 3 Breakdown to inhibit endogenous starch hydrolysis starch intergranular fillers. Stir 3-5 times during soaking;

[0053] 2) The polished rice flour suspension obtained in step 1) is placed on a magnetic stirrer and stirred at a speed of 300 rpm for 4-6 hours under the condition of avoiding light;

[0054] 3) Mix the suspension obtained in step 2) with 4 times the volume of glycerin with a purity ≥ 99.5%, and gently invert up and down and mix repeatedly;

[0055] 4) Put the mixture obtained in step 3) into a centrifuge bottle in a floor centrifuge, set the speed at 2000g / min, and centrifuge for 10min;

[0056] 5) Collect the precipitate and upper liquid obtained in step 4) respectively;

[0057] 6) Wash the precipitate ob...

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Abstract

The invention discloses a method for separating and extracting fillers between rice starch grains. The steps are as follows: 1) Take a sample of polished rice flour in a beaker wrapped in tin foil, add AgNO 3 Soak in the solution to obtain a suspension; 2) Magnetically stir the suspension under dark conditions; 3) Mix it with glycerin to obtain a mixture; 4) Centrifuge the mixture once to obtain a precipitate and supernatant; 5) Wash the primary precipitate with absolute ethanol, remove glycerin, and dry naturally to obtain starch granules; mix the supernatant with absolute ethanol and continue centrifuging to obtain the secondary precipitate; 6) Use absolute ethanol for the secondary precipitate Wash, remove glycerin, and air-dry naturally, which is the crude extract of starch intergranular filler. The method of the present invention has confirmed the feasibility of separating the starch intergranular filler, can effectively separate the starch granule and its granular filler, and the separated intergranular filler crude extract can be used to study the comparison of the structural properties of the starch granule and the intergranular filler, Provide help for analyzing the mechanism of its influence on rice quality.

Description

technical field [0001] The invention belongs to the technical field of rice quality analysis, and in particular relates to a method for separating and extracting fillers between rice starch grains. Background technique [0002] During the rice filling process, as the number and volume of starch granules increase continuously, the starch granules squeeze each other in space. After maturity, most of the starch granules are arranged closely and form a regular polyhedron; The degree of extrusion is not enough to form a spherical or ellipsoidal shape. The protein bodies embedded in these starch granules can inhibit the gelatinization of starch granules. [0003] In addition to the protein, there is also a kind of detritus between the starch grains, which is filled between the starch grains and tightly combined with the starch grains, which cannot be found in the scanning electron microscope of the endosperm cross-section. In addition, such detritus are easily hydrolyzed by thei...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N23/2202
CPCG01N1/28G01N23/2202G01N2223/102G01N2223/07
Inventor 焦桂爱胡培松郭雯唐绍清魏祥进王翠丽
Owner CHINA NAT RICE RES INST