Method for preparing nanoparticles by combining regenerated silk fibroin and photosensitizer

A technology for regenerating silk fibroin and nanoparticles, applied in the fields of chemical drugs and biological applications, can solve the problems of poor stability of micelles and poor biological safety, and achieve the effects of good stability, good dispersibility, and simple and easy method.

Inactive Publication Date: 2021-09-07
SOUTHWEST UNIVERSITY
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Problems solved by technology

[0005] Aiming at the problems of poor stability of micelles and poor biological safety in the current drug delivery system, the present invention provides a method for preparing nanoparticles of regenerated silk fibroin combined with a photosensitizer, in which the photosensitizer chlorinated by a solvent method Phenene e6 is directly incorporated into regenerated silk fibroin by physical entrapment

Method used

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  • Method for preparing nanoparticles by combining regenerated silk fibroin and photosensitizer
  • Method for preparing nanoparticles by combining regenerated silk fibroin and photosensitizer
  • Method for preparing nanoparticles by combining regenerated silk fibroin and photosensitizer

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Embodiment 1

[0021] Example 1 Nanoparticles of regenerated silk fibroin combined with photosensitizer

[0022] according to figure 1 The schematic flow chart of the shown regenerated silk fibroin-bound photosensitizer nanoparticles was prepared as follows:

[0023] (1) Based on silkworm silkworm cocoon degumming and extraction of silk fibroin, the preparation of regenerated silk fibroin RSF solution includes the following steps: first weigh 10g of silkworm silkworm cocoon and place it in 1000ml of sodium carbonate solution with a mass fraction of 0.2%, boil for 30 minutes for degumming, and Stir slowly for 10 minutes, rinse repeatedly with secondary water 2-3 times for purification, and then put the degummed silk fibroin in an oven at 40°C overnight; 2 / absolute ethanol / secondary aqueous solution, add an appropriate amount of degummed silk fibroin, stir for 2-2.5 hours to fully dissolve; filter with 8 layers of gauze, put it into a dialysis bag with a molecular weight cut-off Da=14000,...

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Abstract

The invention particularly relates to preparation of nano-particles based on regenerated silk fibroin combined with a photosensitizer and anti-tumor research of the nano-particles. The method comprises the following synthesis steps: (1) degumming silkworm cocoons to extract silk fibroin, and preparing a regenerated silk fibroin RSF solution; and (2) physically embedding a photosensitizer Ce6 in the silk fibroin by adopting a solvent removal method to prepare the Ce6-loaded silk fibroin nanoparticles SFC NPs. The prepared nanoparticles have uniform size and good stability in an aqueous solution, and have basically negligible toxic and side effects and a good treatment effect in tumor treatment. Meanwhile, near-infrared fluorescence of the photosensitizer Ce6 is used for imaging, and diagnosis and treatment integration is achieved.

Description

technical field [0001] The invention relates to the field of chemical medicine and biological application, in particular to a preparation method of nanoparticles based on regenerated silk fibroin combined with a photosensitizer Ce6 and its application in the delivery of antitumor drugs. Background technique [0002] Chlorin e6 (Chlorin e6, Ce6) is generally synthesized from pheophorbide a, its CAS number: 19660-77-6, chemical structure: C 34 h 36 N 4 o 6 , relative molecular weight: 596.67. Due to the relatively high efficiency of the singlet oxygen generated, it is a good photosensitizer for photodynamic therapy of tumors. However, due to its hydrophobicity, there are certain obstacles in practical operation. [0003] Photodynamic therapy is to use photosensitizers to transmit energy to the surrounding oxygen after being excited under the irradiation of specific wavelengths, generate a large number of ROS, produce cytotoxicity, and thus achieve therapeutic effect on tu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K9/51A61K47/42A61P35/00
CPCA61K41/0071A61K9/5169A61P35/00
Inventor 许志刚许姬明高永娥
Owner SOUTHWEST UNIVERSITY
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