Application of interleukin-5 as marker and/or inhibitor of activity of acute pancreatitis digestive enzyme
A technology for the activity of interleukin and digestive enzymes, applied in the field of medicine, can solve the problem of lack of interleukin-5
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Embodiment 1
[0051] Example 1: Construction of a mouse model of arginine acute pancreatitis
[0052] Twenty g-25g male C57BL / 6 mice (purchased from Hunan Slack Jingda Experimental Animal Co., Ltd.) were fasted overnight and intraperitoneally injected L-arginine hydrochloride 3.3g / kg three times with an interval of 1h between each injection. After the last intraperitoneal injection, the mice were sacrificed at 1h (4), 3h (4), 6h (4), 12h (3), 24h (3) and 48h (2), and their serum was taken for detection .
[0053] Such as figure 2 As shown, the amylase in the serum was at a higher level 1h-24h after the seventh intraperitoneal injection, and decreased at 48h.
[0054] Such as image 3 As shown, the level of lipase in serum gradually increased 1h-6h after the seventh intraperitoneal injection, and gradually decreased 6-48h.
[0055] Such as Figure 4 As shown, the content of interleukin-5 in the serum was at a high level 1h-24h after the seventh intraperitoneal injection, and there was ...
Embodiment 2
[0059] Example 2: Construction of mouse cerulein LPS acute pancreatitis model
[0060] Twenty-seven 20g-25g male C57BL / 6 mice were fasted overnight and injected intraperitoneally with cerulein 50μg / kg for 7 times with an interval of 1 hour. After the last injection of cerulein, LPS was injected intraperitoneally. 1h (4), 3h (4), 6h (4), 12h (4), 24h (4), 48h (4) and 72h (3) after the last injection, the mice were sacrificed , take its serum test.
[0061] Such as Figure 5 and Figure 6 As shown, amylase and lipase in serum reached the highest level 6 hours after the seventh intraperitoneal injection, and then decreased.
[0062] Such as Figure 7 As shown, interleukin-5 in serum was high within 1h-6h after the seventh intraperitoneal injection, and the level decreased thereafter.
[0063] Such as Figure 8 As shown, interleukin-2 in serum was high within 3h-6h after the seventh intraperitoneal injection, and the level decreased thereafter.
[0064] Such as Figure 9 A...
Embodiment 3
[0071] Example 3: Construction of mouse cerulein acute pancreatitis model
[0072] 20g-25g male C57BL / 6 mice were fasted overnight and divided into three groups: normal saline group (5 mice), model group (10 mice), and intervention group (10 mice). The normal saline group was injected with the same volume of normal saline, intraperitoneal injection 7 times with an interval of 1 hour between each time. The model group was intraperitoneally injected 50 μg / kg cerulein 7 times with an interval of 1 hour between each time. The intervention group was injected intraperitoneally with cerulein 50 μg / kg for 7 times with an interval of 1 hour, and interleukin-5 receptor inhibitor was injected intraperitoneally immediately after the first intraperitoneal injection. Twelve hours after the last injection, the mice in the three groups were sacrificed, and their serum was taken for detection.
[0073] Such as Figure 10 and Figure 11 As shown, the activities of amylase and lipase in the ...
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