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Primer and probe for detecting helicobacter pylori and East Asia type of helicobacter pylori

A Helicobacter pylori, East Asian-type technology, applied in the field of biotechnology and diagnostic research, can solve problems such as indistinguishability, and achieve high sensitivity and specificity

Pending Publication Date: 2021-09-21
HANGZHOU NEW HORIZON HEALTH TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this method cannot distinguish CagA-expressing H. pylori, or East Asian strains

Method used

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  • Primer and probe for detecting helicobacter pylori and East Asia type of helicobacter pylori
  • Primer and probe for detecting helicobacter pylori and East Asia type of helicobacter pylori
  • Primer and probe for detecting helicobacter pylori and East Asia type of helicobacter pylori

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0134] PCR reactions were performed using the following items.

[0135] (1) The target to be tested (i.e. the DNA template in the PCR reaction)

[0136] A total of 3 samples, sample A is HP negative, sample B is HP positive, non-East Asian type, and sample C is HP positive, East Asian type.

[0137] (2) Primers

[0138] Choose any one of the forward primers SF1 to SF6, choose SF1 in this example,

[0139] One of the reverse primers SR1 to SR5 is randomly selected, and SR1 is selected in this embodiment.

[0140] (3) Probe

[0141] Any one of the probes SP1 to SP1 is selected, and SP1 is selected in this embodiment.

[0142] The 5' end of the probe is marked with the fluorescent substance Fam, and the 3' end is MGB.

[0143] Fam is carboxyfluorescein, 5-Fam and 6-Fam can be used, preferably 5-Fam.

[0144] MGB is the Minor Groove Binding. The 3′ end of the probe in this embodiment is also connected with a MGB (Minor GrooveBinder) modification group, which can increase the...

Embodiment 2

[0155] PCR reactions were performed using the following items.

[0156] (1) The target to be tested (i.e. the DNA template in the PCR reaction)

[0157] A total of 3 samples, sample A is HP negative, sample B is HP positive, non-East Asian type, and sample C is HP positive, East Asian type.

[0158] (2) Primers

[0159] Choose one of the forward primers F1 to F8 arbitrarily, choose F1 in this embodiment,

[0160] One of the reverse primers R1 to R11 is randomly selected, and R1 is selected in this embodiment.

[0161] (3) Probe

[0162] One of the probes EP1 to EP1 is randomly selected, and EP1 is selected in this embodiment.

[0163] The 5' end of the probe is marked with the fluorescent substance Fam, and the 3' end is MG B.

[0164] Fam is carboxyfluorescein, 5-Fam and 6-Fam can be used, preferably 5-Fam.

[0165] MGB is the Minor Groove Binding. The 3′ end of the probe in this embodiment is also connected with a MGB (Minor GrooveBinder) modification group, which can ...

Embodiment 3

[0176] A kit for detecting Helicobacter pylori and distinguishing East Asian types

[0177] The kit in this example includes: PCR buffer, dNTPs, PCR primers, probes and DNA polymerase.

[0178] Among the PCR primers in this embodiment, the forward primers are F1 and SF1, the reverse primers are R1 and SR1, the probes are SP1 and EP5, the fluorophore at the 5' end of the probe EP5 is FAM, and the fluorophore at the 3' end BHQ1, the fluorescent group at the 5' end of SP1 is CY5, and the fluorescent group at the 3' end is BHQ3.

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Abstract

The invention provides a primer and a probe for detecting helicobacter pylori, and also provides a primer and a probe for distinguishing the East Asian type of helicobacter pylori. The invention also provides a method and a kit for detecting human infected helicobacter pylori and distinguishing the East Asian type of helicobacter pylori by using the primer and the probe disclosed by the invention. The primer and the probe provided by the invention have high sensitivity and specificity, and can distinguish types of HP and detect highly pathogenic HP, the detection method has no invasion step, and targeted treatment can be carried out.

Description

[0001] This application is a divisional application of an invention patent application with an application date of June 2, 2015, an application number of 201510297544.3, and an invention title of "Primers and Probes for Detecting Helicobacter pylori and its East Asian Type". technical field [0002] The invention belongs to the field of biotechnology and diagnostic research, and relates to a primer and a probe for detecting Helicobacter pylori and its East Asian typing, and using the primer and probe to detect whether a sample contains Helicobacter pylori, and to confirm whether it is Methods and kits for typing East Asian types with high risk of pathogenicity. Background technique [0003] Helicobacter pylori (H. pylori or HP for short) is one of the high-risk factors for gastric cancer. This bacterium was found to be associated with gastritis in the early 1980s. After years of research and verification, it was confirmed that H. pylori caused more than 90% of duodenal ulce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/686C12Q1/04C12N15/11C12R1/01
CPCC12Q1/686C12Q1/689C12Q2561/113C12Q2563/107
Inventor 吕宁陈一友沈卫
Owner HANGZHOU NEW HORIZON HEALTH TECH CO LTD