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Haploid SNP (Single Nucleotide Polymorphism) molecular marker related to chalkiness of rice as well as detection method and application thereof

A molecular marker and detection method technology, applied in the field of agricultural molecular biology, to achieve the effect of improving the quality of rice

Active Publication Date: 2021-09-21
HUAZHI RICE BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the production of hybrid rice has reached a relatively high level, there are very few varieties with particularly good rice quality. Therefore, how to quickly and accurately eliminate low-quality rice and select high-quality rice varieties with low chalkiness is the main goal pursued by breeders in the new era.

Method used

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  • Haploid SNP (Single Nucleotide Polymorphism) molecular marker related to chalkiness of rice as well as detection method and application thereof
  • Haploid SNP (Single Nucleotide Polymorphism) molecular marker related to chalkiness of rice as well as detection method and application thereof
  • Haploid SNP (Single Nucleotide Polymorphism) molecular marker related to chalkiness of rice as well as detection method and application thereof

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Embodiment 1

[0027] Example 1 Obtaining of Haplotype SNP Molecular Markers Related to Rice Chalkiness

[0028] 1. Select materials

[0029] The rice materials used in the present invention include natural populations composed of 331 rice germplasm resources, including indica rice and japonica rice, and 29 cultivated rice varieties, wherein the natural populations are from the 3K resources of the International Rice Research Institute.

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Abstract

The invention discloses a haplotype SNP (Single Nucleotide Polymorphism) molecular marker related to chalkiness of rice as well as a detection method and application thereof. The haplotype SNP molecular marker consists of SNP1 and SNP2. The genome position of the SNP1 at the 5365892bp position of the fifth chromosome of the rice genome, and the polymorphism is G or A. The genome position of the SNP2 is at the 5371716bp position of the fifth chromosome of the rice genome, and the polymorphism is G or A. The scheme provided by the invention is beneficial to breeding of rice varieties with low chalkiness, and has important significance for improving the rice quality and cultivating high-quality rice for commercial breeding.

Description

technical field [0001] The invention belongs to the field of agricultural molecular biology, and specifically relates to a haplotype SNP molecular marker related to rice chalkiness, a detection method and application thereof. Background technique [0002] Chalkiness is the white opaque part of the rice endosperm, which is caused by the change in light transmission caused by the gaps between the endosperm starch grains. Chalkiness is the percentage of the projected area of ​​chalky parts in rice to the projected area of ​​the entire rice grain, and high temperature during the filling period, especially at night, is beneficial to the formation of chalkiness). Chalky rice seriously affects the appearance quality, processing quality, eating quality and nutritional quality of rice, and is directly related to the commodity and market value of rice. Highly chalky rice has no market value now. [0003] The chalkiness and transparency of japonica rice are generally better than those...

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 程计华田冰川林鑫李宙炜曾琴贾高峰彭俊华
Owner HUAZHI RICE BIO TECH CO LTD