Centrifugal grinding device for obtaining survival single cells

Abstract

The invention discloses a centrifugal grinding device for obtaining survival single cells. The centrifugal grinding device comprises a centrifugal unit and a vibration grinding unit; the vibration grinding unit is rotationally arranged on the centrifugal unit and comprises a rotating disc; a plurality of sliding grooves are formed in the lower surface of the rotating disc; vibration mechanisms are embedded in the sliding grooves in a sliding mode; the end, close to the circle center of the rotating disc, of any vibration mechanism abuts against the outer side wall of a driving disc, and the circle center of the lower surface of the driving disc is in driving connection with a first driver; and the lower surface of the rotating disc is further provided with return mechanisms which push the vibration mechanisms to abut against and make contact with the driving disc all the time. In the grinding vibration treatment process, space motion formed by combining horizontal reciprocating linear motion, vertical reciprocating linear motion and horizontal rotating motion can be provided at the same time, so that the tissue grinding vibration environment disorder degree is high, the grinding efficiency is high, and the tissue refining period is short.

Description

technical field [0001] The invention relates to the field of biological experimental equipment, in particular to the technical field of biological grinding and extraction tissue processing experimental equipment, and in particular to a centrifugal grinding device for obtaining viable single cells. Background technique [0002] Biological tissue processing is one of the most routine experiments in the laboratory, and various biological experiments need to be carried out on the basis of cell suspensions. With the development of technology, the techniques for biological tissue processing, cell disruption and single-cell processing have also become enriched according to laboratory specifications and requirements. The most common ones are grinding, shaking, and ablation. In addition to the very high cost of ablation technology, conventional grinding and shaking equipment costs are relatively low and can satisfy most laboratory users. [0003] The existing laboratory grinding is ...

AI Technical Summary

Problems solved by technology

[0004] In order to solve the technical problem that it is inconvenient to prepare single cell suspensions and cannot obtain more surviving single cells, this application provides a brand new equipment to replace manual grinding, and at the same time provide centrifugal function, which realizes the integration of grinding and centrifugation, and avoids the existing After manual grinding in the laboratory, it is repeatedly cleaned, and then placed in a separate centrifuge for centrifugation. The present invention is specifically a centrifugal grinding device for obtaining surviving single cells, which can meet the requirements of vibration grinding and centrifugation at the same time. It improves the integration of the equipment and simplifies the operation of the experimenter. At the same time, the vibratory grinding process is more gentle, uniform and balanced than manual grinding, so that the tissue block can be dispersed into single cells, and a greater degree can be obtained. more viable single cells

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