Extraction process of nannochloropsis oculata essential oil
A technique of Nannochloropsis essential oil and an extraction process, which is applied in the field of Nannochloropsis, can solve problems such as affecting the smell of essential oils, and achieves the effects of pure aroma, high purity, and improved release degree
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Embodiment 1
[0027] A kind of extraction technology of Nannochloropsis essential oil, concrete process is as follows:
[0028] (1) Pectinase treatment: mix Nannochloropsis with water, the weight ratio of Nannochloropsis to water is 30:100; stir to disperse Nannochloropsis to obtain a mixed solution; adjust the pH of the mixed solution 4.1, then add pectinase 4.1u / g to it, the enzymolysis temperature is 38°C, and maintain for 4.4h to obtain the enzymolysis solution;
[0029] (2) Collect algae: centrifuge the enzymatic hydrolyzate in step (1), collect the solid, and then wash the solid three times to obtain the algae; through centrifugation, the cell structure of Nannochloropsis is not destroyed. The colloid after enzymatic hydrolysis is filtered to avoid the impact of the colloid on the smell of essential oils;
[0030] (3) Juice extraction: use the freeze-thaw method to freeze and thaw the algae body three times, and then press the algae body to obtain the algae liquid; the process of the...
Embodiment 2
[0035] A kind of extraction technology of Nannochloropsis essential oil, concrete process is as follows:
[0036] (1) Pectinase treatment: mix Nannochloropsis with water, the weight ratio of Nannochloropsis to water is 20:100; stir to disperse Nannochloropsis to obtain a mixed solution; adjust the pH of the mixed solution 4.2, then add pectinase 3.5u / g to it, enzymolysis temperature is 35°C, keep for 4h to obtain enzymolysis solution;
[0037] (2) Collect algae: centrifuge the enzymatic hydrolyzate in step (1), collect the solid, and then wash the solid three times to obtain the algae; through centrifugation, the cell structure of Nannochloropsis is not destroyed. The colloid after enzymatic hydrolysis is filtered to avoid the impact of the colloid on the smell of essential oils;
[0038] (3) Juice extraction: use the freeze-thaw method to freeze and thaw the algae body three times, and then press the algae body to obtain the algae liquid; the process of the freeze-thaw metho...
Embodiment 3
[0043] A kind of extraction technology of Nannochloropsis essential oil, concrete process is as follows:
[0044] (1) Pectinase treatment: mix Nannochloropsis with water, the weight ratio of Nannochloropsis to water is 40:100; stir to disperse Nannochloropsis to obtain a mixed solution; adjust the pH of the mixed solution 4.3, then add pectinase 4.3u / g to it, the enzymolysis temperature is 40°C, and keep for 5h to obtain the enzymolysis solution;
[0045] (2) Collect algae: centrifuge the enzymatic hydrolyzate in step (1), collect the solid, and then wash the solid three times to obtain the algae; through centrifugation, the cell structure of Nannochloropsis is not destroyed. The colloid after enzymatic hydrolysis is filtered to avoid the impact of the colloid on the smell of essential oils;
[0046] (3) Juice extraction: use the freeze-thaw method to freeze and thaw the algae body three times, and then press the algae body to obtain the algae liquid; the process of the freez...
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