Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of CEBP alpha as RANBP2 target site in preparation of glycosylation medicine for treating liver cancer

A technology for the treatment of liver cancer and glycosylation, which is applied in the field of biomedicine and can solve problems such as decreased expression level

Active Publication Date: 2021-10-15
THE THIRD XIANGYA HOSPITAL OF CENT SOUTH UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous studies have found that the level of O-glycosylation modification in HCC is significantly higher than that in normal tissues and adjacent tissues, while the expression level of OGA protein is generally decreased.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of CEBP alpha as RANBP2 target site in preparation of glycosylation medicine for treating liver cancer
  • Application of CEBP alpha as RANBP2 target site in preparation of glycosylation medicine for treating liver cancer
  • Application of CEBP alpha as RANBP2 target site in preparation of glycosylation medicine for treating liver cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0029] The present invention will be further explained and illustrated below in conjunction with the accompanying drawings and experimental data.

[0030] , materials and methods

[0031] Cell culture and transfection, qRT-PCR analysis, Western blot analysis, immunohistochemical assay, MTT assay, flow cytometry, immunoprecipitation, chromatin immunoprecipitation, immunofluorescence, dual luciferase assay, flow cytometry For the determination of Lipid-ROS, the above methods are all existing methods and will not be repeated here.

[0032] ,result

[0033] 2.1 RANBP2 is highly expressed in liver cancer tissues, which can cause the decrease of OGA expression

[0034] We first measured the expression of RANBP2 in 50 cases of clinical cancer tissues and adjacent tissues by immunohistochemistry and Western blot, and found that the immunohistochemical score of RANBP2 in liver cancer tissues was significantly higher than that in corresponding adjacent tissues ( figure 1 A, 1B). Cel...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses application of CEBP alpha as a detection reagent of an RANBP2 target site in preparation of glycosylation related medicine for treating liver cancer. Researches show that high expression of RANBP2 in liver cancer cells is positively correlated with liver cancer malignant phenotypes, and RANBP2 down-regulates OGA expression and increases O-glycosylation modification, so that proliferation and invasion abilities of the liver cancer cells are improved. As a key transcription factor CEBP alpha for forward regulation of OGA, RANBP2 can induce SUMOylation of the CEBP alpha, and experiments prove that the K196 site of the CEBP alpha is the SUMO key target site of the RANBP2, and the SUMOylation of the site can promote CEBP alpha protein degradation. OGA-mediated O-glycosylation pathways can be slowed down by protecting the K196 site of the CEBP alpha from being influenced by SUMOylation modification or improving the expression of the CEBP alpha.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to the application of transcription factor CEBPα as a target of RANBP2 in the diagnosis and treatment of glycosylation-related drugs in hepatocellular carcinoma. Background technique [0002] Hepatocellular carcinoma (HCC) is a malignant tumor that seriously plagues and threatens the health of modern people, accounting for 18.8% of the total number of malignant tumor deaths. Although surgical resection, liver transplantation, postoperative radiotherapy and chemotherapy, and molecular targeted therapy have made great progress in the treatment of liver cancer, the overall survival rate of liver cancer patients has not improved significantly compared to before. Hepatocellular carcinoma has the characteristics of high recurrence rate, high mortality rate, and poor prognosis. The reason is that the early diagnosis and examination methods of liver cells are not standardized...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61P35/00
CPCA61K45/00A61P35/00
Inventor 曹科刘晓明陈兴宇
Owner THE THIRD XIANGYA HOSPITAL OF CENT SOUTH UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com