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TRF related to pancreatic cancer and application thereof

A positive correlation technology for pancreatic cancer, applied in the field of pancreatic cancer, can solve the problems of resistance to chemotherapy drugs, lack of predictive index treatment options for pancreatic cancer, and poor prognosis

Active Publication Date: 2021-03-19
SUN YAT SEN UNIV CANCER CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemotherapy drug resistance is prone to occur, and the prognosis is extremely poor
However, there is no report that tRFs regulate the occurrence and development of pancreatic cancer.
[0004] At present, pancreatic cancer still lacks more accurate predictors and effective treatment options.

Method used

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  • TRF related to pancreatic cancer and application thereof
  • TRF related to pancreatic cancer and application thereof
  • TRF related to pancreatic cancer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Correlation between tRF-21-VBY and clinical data of pancreatic cancer patients

[0036] 1. Collection of samples

[0037] A total of 227 pairs of cancer and paracancerous tissues were collected from Guangzhou and Beijing pancreatic cancer cohorts, total RNA was extracted, reverse transcription was performed using tRF-specific reverse transcription primers, and tRF-21-VBY (its nucleotide The sequence is expressed as shown in SEQ ID No.1), and U6 is used as an internal reference. Specific primers are listed in Table 1.

[0038] The PCR primers of table 1 tRF-21-VBY reverse transcription primers, PCR primers and U6:

[0039]

[0040] 2. tRF-21-VBY is significantly associated with better prognosis in patients with pancreatic cancer

[0041] 1. Experimental method

[0042] The relationship between high and low expression of tRF-21-VBY and the survival time of 158 patients with pancreatic cancer in Guangzhou cohort was analyzed by Kaplan-Meier method.

[004...

Embodiment 2

[0050] Example 2 Effect of tRF-21-VBY on malignant phenotype of pancreatic cancer cells

[0051] 1. In vitro experiment

[0052] 1. Experimental method

[0053] The synthetic tRF-21-VBY (its nucleotide sequence as shown in SEQ ID No.1) or its antisense sequence were inserted into the pLKD-CMV-mcherry-2A-Neo-U6-shRNA vector respectively to construct stable Overexpression and knockdown of pancreatic cancer cell lines, in vitro experiments to investigate the changes in the malignant phenotype of pancreatic cancer cells.

[0054] CCK8 kit was used to detect the proliferation ability of cells. Pancreatic cancer cells were planted in 96-well plates and cultured continuously at 37°C. CCK8 detection reagent was added to the medium at specific intervals, and the absorbance value of cells was recorded by a microplate reader. The proportion of apoptotic tumor cells was detected by flow cytometry. All the pancreatic cancer cells planted in the cell culture dish were digested and collect...

Embodiment 3

[0062] Example 3 Experimental treatment of tRF-21-VBY in pancreatic cancer animal models

[0063] 1. Experimental method

[0064] Using Luciferase-labeled pancreatic cancer cells that stably knocked down tRF-21-VBY, three animal models including pancreatic cancer orthotopic transplantation model, lung metastasis model and PDX were constructed. 2×10 6 A pancreatic cancer cell stably knocking down tRF-21-VBY was surgically inoculated in the pancreatic tissue of mice, and the mice were randomly divided into two groups 7 days later. 1×10 6 A pancreatic cancer cell stably knocking down tRF-21-VBY was injected into the mice through the tail vein to colonize the lungs. On the second day, the mice were randomly divided into two groups. Pancreatic cancer tissues from 3 pancreatic cancer patients were planted subcutaneously in immunodeficient mice for passage. When the passage reached the third generation (F3), when the tumor grew to 200mm 3 , were randomly divided into 2 groups.

...

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PUM

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Abstract

The invention discloses tRF related to pancreatic cancer. The nucleotide sequence of the tRF related to pancreatic cancer is shown as SEQ ID No. 1. The present invention finds a tRNA-derived fragmenttRF-21-VBY having a function of highly expressing a malignant phenotypic function that significantly inhibits pancreatic cancer cells, which has the ability to inhibit cell proliferation, migration and invasion, promotes apoptosis, inhibits subcutaneous transplantation tumor growth and pancreatic cancer in-situ tumor growth, and can indicate the prognosis of pancreatic cancer patients. Therefore,the tRF-21-VBY or the activator thereof can be used for preparing a medicine for treating pancreatic cancer or screening a medicine suitable for treating pancreatic cancer; the reagent for detecting tRF-21-VBY can be used for preparing a pancreatic cancer prognosis prediction kit.

Description

technical field [0001] The present invention relates to the technical field of pancreatic cancer, more specifically, to a tRF related to pancreatic cancer and its application. Background technique [0002] Pancreatic cancer is a malignant tumor of the digestive system, with a 5-year survival rate of less than 9%. Many pancreatic cancers are diagnosed at an advanced stage and have lost the chance of being cured by surgery. Chemotherapy drug resistance is prone to occur, and the prognosis is extremely poor. It is prone to local invasion, and can also be transferred to distant organs through lymphatic and hematogenous. Therefore, elucidating the underlying mechanism of pancreatic cancer development and finding new therapeutic targets is crucial for the treatment of pancreatic cancer. [0003] In recent years, the research on non-coding RNA and tumor is very extensive, and it plays a very important role in the occurrence and development of tumor. tRNA-derived fragment (tRNA-...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12Q1/6886A61K31/7088A61K45/00A61P35/00A61P35/04
CPCC12N15/113C12Q1/6886A61K31/7088A61K45/00A61P35/00A61P35/04C12Q2600/118C12Q2600/136C12Q2600/158C12Q2600/178
Inventor 林东昕郑健泮玲
Owner SUN YAT SEN UNIV CANCER CENT
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