Bacillus cereus and application thereof

A technology of Bacillus cereus and functional bacterial agent, applied in Bacillus cereus and its application fields, can solve the problems of host poisoning, achieve the effects of inhibiting growth and reproduction, high application value, and improving diarrhea

Inactive Publication Date: 2021-10-15
ANHUI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, not all Bacillus strains are suitable for making probiotics, and some strains may produce diarrhea-causing toxins and cause toxic effects on the host

Method used

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  • Bacillus cereus and application thereof
  • Bacillus cereus and application thereof
  • Bacillus cereus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: Cultivation and identification of Bacillus cereus HMPM18123 in the example of the present invention. Bacillus cereus HMPM18123, preservation unit: Guangdong Microbial Culture Collection Center, preservation place: 5th Floor, No. 59, Compound, No. 100 Xianlie Middle Road, Guangzhou City, preservation number: GDMCC 61838.

[0048] The culture method of the Bacillus cereus HMPM18123 bacterial strain of the embodiment of the present invention:

[0049] The aerobic liquid culture of Bacillus cereus uses LB liquid medium. The specific formula is 10g tryptone, 5g yeast extract and 10g NaCl, distilled water to 1000mL, and sterilized at 121°C for 20min. Before culturing, the preserved strains were inoculated into LB liquid medium at a ratio of 1%, activated at 37°C and 120rpm for 12h, and then inserted into LB liquid medium at 37°C and 120rpm with a 1% inoculation amount for cultivation.

[0050] Aerobic solid culture of Bacillus cereus uses LB solid medium. The sp...

Embodiment 2

[0059] Example 2: Survival rate of Bacillus cereus HMPM18123 simulated gastrointestinal fluid in vitro

[0060] Experimental method: simulated artificial gastric juice (SGF): prepared by dissolving NaCl (32.0 g) and pepsin (2 g) in 1 L of deionized water, and adjusting the pH to 2.0 with dilute hydrochloric acid solution.

[0061] Simulated Artificial Intestinal Fluid (SIF): by adding KH 2 PO 4 (6.8g) was dissolved in 250mL of deionized water, and then 77mL of 0.2mol / L NaOH solution, 200mL of deionized water and trypsin (10g) were added. The pH of the mixture was adjusted to 6.8 with NaOH solution.

[0062] After the preserved strains were activated at 37°C for 12 hours, they were inoculated into SGF and SIF at an inoculation amount of 1%, and cultured at 37°C. The bacterial solution was taken out at 0h, 1h, 2h and 3h, diluted in different proportions, and 100 μL of the bacterial solution was spread on the LB solid plate, and the colony count was performed after 10h.

[00...

Embodiment 3

[0064] Example 3: Bacillus cereus has no toxic side effects on C57BL / 6J mice

[0065] Bacillus cereus was resuspended in normal saline to make a concentration of 1×10 9 CFU / mL bacterial suspension. Take 10 healthy male C57BL / 6J mice with a body weight of 20±2g, and after acclimating to the environment for 1 week, each mouse was given 2×10 8 The CFU bacterial suspension was gavaged once, observed for a week, and the death and body weight were recorded.

[0066] The results are shown in Table 1, the feeding concentration was 2×10 8 CFU of Bacillus cereus had no significant impact on mice, no significant changes in body weight, and no death.

[0067] Table 1 Changes in body weight and death of mice after intragastric administration of Bacillus cereus for 1 week

[0068]

[0069] Note: —: No mice died.

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Abstract

The invention relates to bacillus cereus, the scientific name of the bacillus cereus is Bacillus cereus HMPM18123, the preservation date is July 30, 2021, the preservation unit is Guangdong Microbial Culture Collection Center, the preservation place is the 5th floor, No.59, No.100 Courtyard, Xianlie Middle Road, Guangzhou, and the preservation code is GDMCC61838. The invention discloses application of bacillus cereus in preparation of functional microbial agents, food and/or medicines for improving ulcerative colitis, reducing pathological injury of enteritis intestinal tissues, inhibiting inflammation, improving the barrier function of intestinal tracts and improving intestinal flora composition. The traditional Chinese medicine composition has the advantages of high practical application value and small toxic and side effects, and has a very obvious effect on treating ulcerative colitis.

Description

technical field [0001] The invention relates to the technical field of bacillus cereus, in particular to a bacillus cereus and application thereof. Background technique [0002] Ulcerative colitis (UC) is a major type of inflammatory bowel disease (inflammatory bowel disease, IBD), which is a chronic non-specific intestinal inflammation of unknown cause. In recent years, the number of patients with UC in China has shown an obvious upward trend. UC lesions are mainly located in the colonic mucosa and submucosa; the clinical manifestations are diarrhea, mucus pus and blood in the stool, abdominal pain, tenesmus, etc., and most of them are chronic and recurrent. The current treatment methods for UC mainly include drug therapy, nutrition therapy, psychotherapy and surgical treatment, among which drug therapy is the most important method. The drugs used clinically to treat UC mainly include aminosalicylic acid drugs, adrenal glucocorticoids and immunosuppressants. However, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/742A61P1/04A61P1/00A23L33/135C12R1/085
CPCC12N1/20A61K35/742A61P1/04A61P1/00A23L33/135A23V2002/00A23V2200/3204
Inventor 王永中汪静敏安景张光辉许一凡盛康亮
Owner ANHUI UNIVERSITY
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