Sex hormone dissociation agent and detection kit

A detection kit and technology for sex hormones, which is applied in the field of immunoassays, can solve the problems of easy cross-reaction of sex hormones, reduction of detectable hormone concentrations, and inability to distinguish by immunoassay methods, so as to save sample consumption, reduce workload and labor time, Avoid the effect of repeated processing

Pending Publication Date: 2021-10-15
北京乐普诊断科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, the immunoassay method is mainly used for the determination of sex hormones in human body. Due to the interference of different endogenous compounds, the immunoassay method cannot distinguish sex hormones and is prone to cross-reactions. Exogenous hormones will also interfere with the test results and lead to overestimation of the results. , most of the hormones in the human body are easily combined with proteins, reducing the concentration of detectable hormones. Direct immunoassays can only detect concentrations as low as 30-40pg / ml, which is far from meeting the needs of children, menopausal women, men, Measurement needs in women treated with aromatase preparations

Method used

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  • Sex hormone dissociation agent and detection kit
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  • Sex hormone dissociation agent and detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] This example is a comparative example of the technical solution of the present invention. The provided detection kit is based on magnetic particle chemiluminescence method, and the detection kit includes sample diluent, magnetic particle reagent and antibody reagent.

[0032] The preparation method of the detection kit is as follows:

[0033] 1. Solution preparation

[0034]Preparation of activation solution: Weigh 10.66g of activator MES into a beaker, add 1L of purified water, stir and mix well, adjust the pH to 6.0 with NaOH solution, and store at 4°C for later use.

[0035] Preparation of coupling solution: weigh 2.865g of Na 2 HPO 4 12H 2 O in a beaker, weigh 0.272g of KH in turn 2 PO 4 ·H 2 O, 7.948g of NaCl and 1.983g of KCl were added to the above beaker, made up to volume with 1L of purified water, stirred and mixed, adjusted to pH 7.4, and stored at 4°C for later use.

[0036] Preparation of sample diluent: Prepare 0.1M Tris buffer, pH8.0, containing 0....

Embodiment 2

[0045] The kit provided in this example is based on the magnetic particle chemiluminescence method, and the kit includes a dissociating agent, a magnetic particle reagent and an antibody reagent. Among them, the dissociating agent contains 0.1%~5% w / v sensitizer, 0.05%~10% w / v dissociating substance, 0.01%~0.5% w / v surfactant, 0.5%~5% w / v Protective agent and 0.02% to 0.2% w / v preservative; magnetic particle reagent adopts chemical cross-linking method to make estradiol, estriol, progesterone, Dehydroepiandrosterone sulfate and testosterone (or corresponding derivatives) are coupled with magnetic microspheres to prepare corresponding magnetic particle reagents; antibody reagents are formed by combining antibodies with acridinium esters.

[0046] The preparation method of the kit is as follows:

[0047] 1. Solution preparation

[0048] Preparation of activation solution: Weigh 10.66g of activator MES into a beaker, add 1L of purified water, stir and mix well, adjust the pH to...

Embodiment 3

[0059] The kit provided in this example is based on the magnetic particle chemiluminescence method, and the kit includes a dissociating agent, a magnetic particle reagent and an antibody reagent. The preparation method of the kit is as follows:

[0060] 1. Solution preparation

[0061] Preparation of activation solution: Weigh 10.66g of activator MES into a beaker, add 1L of purified water, stir and mix well, adjust the pH to 6.0 with NaOH solution, and store at 4°C for later use.

[0062] Preparation of coupling solution: weigh 2.865g of Na 2 HPO 4 12H 2 O in a beaker, weigh 0.272g of KH in turn 2 PO 4 ·H 2 O, 7.948g of NaCl and 1.983g of KCl were added to the above beaker, made up to volume with 1L of purified water, stirred and mixed, adjusted to pH 7.4, and stored at 4°C for later use.

[0063] Preparation of dissociation agent: prepare 0.1M Tris buffer, pH8.0, containing 0.2M NaCl, 1% (w / v) PEG6000, 0.5% (w / v) sucrose, 0.1% octylphenol polyoxyethylene Ether, 0.3% ...

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Abstract

The invention relates to a sex hormone dissociation agent and a detection kit, the sex hormone dissociation agent is a component used in cooperation with sex hormone detection, and the sex hormone dissociation agent comprises 0.1%-5% w / v of a sensitizer, 0.05%-10% w / v of hormone, 0.01%-0.5% w / v of a surfactant and a liquid reagent. According to the sex hormone dissociation agent, estradiol, estriol, progesterone, dehydroepiandrosterone sulfate and testosterone which are combined on protein can be separated without influencing normal detection of the hormones, so that the detection capability of the reagent is improved, and the agent has the advantages of high dissociation speed, high efficiency and no influence on the reaction performance of the reagent; the important guarantee is provided for improving the sex hormone detection level and accuracy.

Description

technical field [0001] The invention relates to the field of immune detection, in particular to a sex hormone dissociating agent and a detection kit. Background technique [0002] At present, the immunoassay method is mainly used for the determination of sex hormones in human body. Due to the interference of different endogenous compounds, the immunoassay method cannot distinguish sex hormones and is prone to cross-reactions. Exogenous hormones will also interfere with the test results and lead to overestimation of the results. , most of the hormones in the human body are easily combined with proteins, reducing the concentration of detectable hormones. Direct immunoassays can only detect concentrations as low as 30-40pg / ml, which is far from meeting the needs of children, menopausal women, men, Measuring needs in women treated with aromatase preparations. [0003] Therefore, a technical problem urgently needed to be solved by those skilled in the art is: how to provide a sa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/74G01N33/543
CPCG01N33/743G01N33/74G01N33/54326
Inventor 张勇苏义敬李文娜
Owner 北京乐普诊断科技股份有限公司
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