Method of obtaining lipids from microbial cell composition by enzyme and ph shock

A microbial cell and composition technology, applied in the direction of microorganism-based methods, microorganism dissolution, biochemical equipment and methods, etc., can solve a large number of cost and time problems, reduce monetary costs, reduce processing time, and reduce variability Effect

Pending Publication Date: 2021-10-22
DSM IP ASSETS BV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in some embodiments, this method requires multiple washing steps, which require significant cost and time

Method used

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  • Method of obtaining lipids from microbial cell composition by enzyme and ph shock

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0169] Embodiment 1. Pilot test (AEX-O-1150-tank B)

[0170] 524.6 kg of fermentation broth containing about 10% w / w of Schizochytrium microbial cells was heated to 60°C and the pH was adjusted by adding 50% NaOH (available from Colonial Chemicals Solutions, 916 W Lathrop Ave, Savannah, GA 31415). Adjust to 8.3. The amount of 0.3% based on the weight of the culture medium 2.4 FG (available from Novozymes, Franklinton, N.C.) was added to the broth for enzymatic cleavage, and after 30 minutes the pH drifted to 6.9 and readjusted to 8.1. After 2 hours of enzymatic cleavage, the demulsification process was started by raising the pH to 10.5 and the temperature to 90°C. When the temperature reached 90°C, the pH drifted to 8.0 and readjusted to 10.2. After breaking the emulsion for 2 hours, 10.49 kg of NaCl salt were added. Thirty minutes after salt addition, the pH was lowered to 5.4 by adding 50% citric acid (prepared in house from solid citric acid (available from Tate & Lyle...

Embodiment 2

[0171] Embodiment 2. Pilot test (AEX-O-1150-tank C)

[0172] 525.2 kg of the fermented liquid containing about 10% w / w of the Schizochytrium microbial cells (the fermented liquid of the same batch as tank B in Example 1) was heated to 60° C., and added 50% NaOH (can be Obtained from Colonial Chemicals Solutions, 916W Lathrop Ave, Savannah, GA 31415) the pH was adjusted to 7.9. The amount of 0.3% based on the weight of the culture medium 2.4 FG (available from Novozymes, Franklinton, N.C.) was added to the culture medium for enzymatic cleavage. After 30 minutes, the pH drifted to 6.7 and readjusted to 8.1. After 2 hours of enzymatic cleavage, the demulsification process was started by raising the pH to 10.5 and the temperature to 90°C. When the temperature reached 90°C, the pH was readjusted to 10.3. After 1.5 hours of demulsification, 10.5 kg of NaCl salt were added. After 30 minutes of salt addition, the temperature was lowered to 85°C and the pH was lowered to 5.5 by a...

Embodiment 3

[0178] Embodiment 3. pilot test

[0179] 1797.6 kg of fermentation broth containing about 10% w / w of Schizochytrium microbial cells was heated to 60°C and the pH was adjusted by adding 50% NaOH (available from Colonial Chemicals Solutions, 916W Lathrop Ave, Savannah, GA 31415). Adjust to 8.0. The amount of 0.3% based on the weight of the culture medium 2.4 FG (available from Novozymes, Franklinton, N.C.) was added to the medium for enzymatic cleavage for two hours. The pH of the lysed composition was adjusted to 10.5 and heated to 90°C. When the temperature reached 90°C, the pH drifted to 8.8, and the pH was adjusted to 10.1. After 1 hour at 90°C, the temperature was lowered to 82°C and the pH was lowered by adding 50% citric acid (prepared in house from solid citric acid (available from Tate & Lyle, 2200E Eldorado St, Decatur Illinois USA, 62521)). to 5.2 for 30 minutes. The pH was then raised to 10.1 for 40 minutes and the pH was again lowered to 5.4. After 30 minutes ...

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Abstract

The present invention is directed to a process for obtaining a lipid from a cell, the process involving lysing a cell to form a lysed cell composition; optionally adding a salt to the lysed cell composition; sequentially, in one or more cycles, raising the pH of the lysed cell composition to 10 or above for a period of time, followed by lowering the pH of the lysed cell composition to a pH of less than 6 for a period of time to demulsify the cell composition; and separating a lipid from the demulsified cell composition.

Description

[0001] Cross References to Related Applications [0002] This application is an international application claiming priority to U.S. Provisional Application No. 62 / 818563, filed March 14, 2019, and U.S. Provisional Application No. 62 / 818944, filed March 15, 2019, each of which The entire content of the author is hereby incorporated by reference in its entirety. technical field [0003] The present invention relates to methods for obtaining lipids from compositions comprising microbial cells by lysing the cells, sequentially raising and lowering the pH of the cells for one or more cycles, and isolating the lipids. The invention also relates to lipids prepared by the methods of the invention. Background technique [0004] A typical method for obtaining lipids, such as polyunsaturated fatty acids, from microbial cells involves growing microorganisms capable of producing the desired lipids in a fermenter, pond, or bioreactor; a fermentation broth of a substance; drying said mi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/10C11B3/02C11B3/16
CPCC11B3/16A23D9/02C11B1/02C11B1/025C11B3/04C11B3/06C12P7/6463C12P7/6472C12Y304/21C12R2001/645C12N1/06C11B3/02
Inventor 内加什·阿杜格纳
Owner DSM IP ASSETS BV
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