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Micro-nano silk fibroin-induced bone mineralization calcium phosphate-based bone cement and preparation method thereof

A technology of silk fibroin and calcium phosphate, which is applied in the field of biomedical materials, can solve problems such as limitations, slow degradation speed, and low mechanical properties, and achieve good self-curing properties, promote adhesion, and promote the effects of mineral deposition

Active Publication Date: 2022-05-17
TAIYUAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Its self-curing, plasticity, non-toxic degradation products, and the ability to convert into hydroxyapatite-like properties after hydration make it one of the main components of calcium phosphate bone cement, but its degradation speed in the human body It is too slow, and it takes more than two years to completely degrade. In addition, the longer setting time and lower mechanical properties also limit its application

Method used

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  • Micro-nano silk fibroin-induced bone mineralization calcium phosphate-based bone cement and preparation method thereof
  • Micro-nano silk fibroin-induced bone mineralization calcium phosphate-based bone cement and preparation method thereof
  • Micro-nano silk fibroin-induced bone mineralization calcium phosphate-based bone cement and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Preparation of α-calcium sulfate hemihydrate: Put 80 g calcium sulfate dihydrate in a closed autoclave, heat to 120°C, the pressure is about 0.138MPa at this time, keep the pressure and heat at constant temperature for 9 hours. After that, the material was taken out and placed in an oven at 110°C to dry for 2 hours, then cooled and ground, and sieved through a 200-mesh sieve to obtain α-calcium sulfate hemihydrate powder with uniform particle size.

[0027] Preparation of α-tricalcium phosphate: react 1000ml 0.26mol / L phosphoric acid solution with 0.13mol calcium carbonate for 1.5h to prepare solution C, slowly add solution C dropwise to 1000ml 0.13mol / L calcium hydroxide suspension , fully reacted for 4 hours to obtain suspension D; then added 0.13mol calcium carbonate and suspension D to react overnight, and obtained tricalcium phosphate precursor through aging, centrifugation, ball milling and other steps; after vacuum drying, sintering, grinding, Sieve and other ste...

Embodiment 2

[0033] The preparation of α-type calcium sulfate hemihydrate, drug-loaded silk fibroin microspheres, and mineralized silk fibroin nanofiber solutions is the same as in Example 1.

[0034] Mix 0.09g mineralized silk fibroin, 0.1455g α-calcium sulfate hemihydrate, and 2.7645g α-tricalcium phosphate, and add 1.2ml of 0.4wt% nano-silk fibroin fiber solution to prepare α-tricalcium phosphate / α-calcium sulfate hemihydrate / mineralized silk fibroin / nano silk fibrous bone repair material.

[0035] The final setting time of calcium phosphate-based bone cement materials induced by micro-nano silk fibroin in bone mineralization was 18min~20min, the compressive strength was 4.29MPa~6.62MPa, the average weight loss rate was 8.18% after 2 weeks of degradation, and the degradation rate was 4 The average weight loss rate after one week was 9.78%.

Embodiment 3

[0037] The preparation of α-type calcium sulfate hemihydrate, drug-loaded silk fibroin microspheres, and mineralized silk fibroin nanofiber solutions is the same as in Example 1.

[0038]Mix 0.09g mineralized silk fibroin, 0.291g α-calcium sulfate hemihydrate, and 2.619g α-tricalcium phosphate, and add 1.2ml of a 0.4wt% nano-silk fibroin fiber solution to prepare α-tricalcium phosphate / α-calcium sulfate hemihydrate / mineralized silk fibroin / nano silk fibrous bone repair material.

[0039] The final setting time of the calcium phosphate-based bone cement material induced by micro-nano silk fibroin in bone mineralization was 15min~18min, the compressive strength was 7MPa~7.25MPa, the average weight loss rate was 1.87% after 2 weeks of degradation, and the average weight loss rate was 1.87% after 4 weeks of degradation The average weight loss rate was 4.42%.

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Abstract

Micro-nano silk fibroin-induced bone mineralization calcium phosphate-based bone cement and its preparation method belong to the field of biomedical materials. , 3wt% of micron-scale mineralized silk fibroin is mixed uniformly and then added to a solution of nano-scale silk fibroin fibers at a liquid-solid ratio of 0.4ml / g. The invention obviously improves the compressive strength of the pure α-tricalcium phosphate bone cement, up to 11.48MPa, and can promote the deposition of hydroxyapatite at the same time. After the bone repair material was degraded for 4 weeks, when the proportion of α-calcium sulfate hemihydrate was 0wt%~25wt%, the degradation weight loss rate was within ‑0.18%~12.08%, indicating that the degradation process of α-calcium sulfate hemihydrate At the same time, along with the conversion of α-tricalcium phosphate to hydroxyapatite minerals, the pore structure formed by the degradation of α-calcium sulfate hemihydrate also further promotes mineral deposition, and at the same time provides space for cell migration and growth, so that the bone around the defect Trabeculae grow in, eventually forming new bone tissue and completing bone regeneration.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a calcium phosphate-based bone cement induced by micronano silk fibroin and bone mineralization and a preparation method thereof. Background technique [0002] At present, critical size bone defects caused by traffic accidents, trauma, and myeloma surgery are beyond the scope of the human body's own repair ability. In order to better repair bone defects, it is very important to find a biomedical degradable bone repair material. [0003] α-Tricalcium phosphate has received extensive attention since Brown and Chow proposed the concept of calcium phosphate bone cement in 1978. Its self-curing, plasticity, non-toxic degradation products, and the ability to convert into hydroxyapatite-like properties after hydration make it one of the main components of calcium phosphate bone cement, but its degradation speed in the human body It is too slow, and it takes more than two...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/02A61L27/12A61L27/22A61L27/50A61L27/58D01F4/00
CPCA61L27/025A61L27/12A61L27/227A61L27/58A61L27/50D01F4/00A61L2430/02A61L2400/12A61L2400/06C08L89/00
Inventor 连小洁宋亚萍梁俊杰赵鸿运牛宝龙黄棣
Owner TAIYUAN UNIV OF TECH
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