Preparation method and application of anti-aging and anti-wrinkle yeast extract

A technology of yeast extract and Saccharomyces cerevisiae, which is applied in the field of preparation of yeast extract, can solve the problems of high cost, complicated preparation process, high quality standard, etc., and achieve the effect of precise classification and positioning, broad market prospect and huge economic value

Pending Publication Date: 2021-11-02
广州暨创生物医药研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 1. The efficacy of yeast extract is complete but not precise, there is no very clear focus on anti-aging and anti-wrinkle, and it is difficult to meet the precise classification and positioning of users' needs for cosmetics;
[0006] 2. The composition of yeast extract is complex, and most manufacturers cannot clearly and clearly label its specific composition and content, and clarify the specific mechanism of its efficacy;
[0007] 3. The quality standards of these two types of raw materials used in the field of cosmetics are high, the preparation process is complicated, and the cost is high;
[0008] 4. There are still disputes over transdermal absorption and excessive proliferation of growth factor peptide raw materials used in skin care products;
[0009] 5. Growth factor EGF (Epidermal Growth Factor) has been clearly excluded from the raw materials that can be used in cosmetics. It is difficult for skin care products containing growth factor EGF to obtain approval for production and sales, and it is also difficult to be accepted by consumers

Method used

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  • Preparation method and application of anti-aging and anti-wrinkle yeast extract
  • Preparation method and application of anti-aging and anti-wrinkle yeast extract
  • Preparation method and application of anti-aging and anti-wrinkle yeast extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0131] Embodiment 1 mutagenizes industrial Saccharomyces cerevisiae strains by ARTP mutagenesis method

[0132] 1. Activation and fermentation of industrial Saccharomyces cerevisiae

[0133] Starting strain: Industrial Saccharomyces cerevisiae (Saccharomyces cerevisiae, ATCC 9080);

[0134] The Saccharomyces cerevisiae strain was inserted into the fermentation medium, and cultured with shaking at 30°C and 150r / min until the bacteria grew to the logarithmic phase.

[0135] 2. Using the ARTP mutagenesis method to mutate Saccharomyces cerevisiae strains

[0136] Take 1 mL of the cultured bacterial solution to the mid-logarithmic phase, put it in a 1.5 mL EP tube, centrifuge at 8000 r / min at 4 °C for 5 min, and discard the supernatant;

[0137] After washing 3 times with normal saline containing glycerol (mass fraction 5%), draw 10 μL of the suspension and place it on a cell counting plate, count the number of bacteria with a microscope, and dilute the bacteria to a concentratio...

Embodiment 2

[0166] Example 2 Purification of Yeast Extract by Molecular Sieve and Heparin Affinity Chromatography

[0167] 1. Yeast cell treatment

[0168] Use resuspension (20mM Tris-HCl, 0.1M NaCl, pH 7.0) to resuspend JCY-P-998 bacteria according to the wet weight of the bacteria (g): resuspension (mL) = 1:15 (10000rpm, 3min ), tissue mashing homogenizer (high pressure homogenization 800bar) 2 times of homogenization;

[0169] Insert the probe into the homogenate, about 3cm away from the bottom of the container, set the power at 10%, sonicate for 3s each time, with an interval of 5s, sonicate for 20min, and sonicate for 3 times. This process is carried out in an ice bath;

[0170] After the bacteria are broken, use a large-scale low-temperature centrifuge at 4°C, centrifuge at 17,000g for 30min, and collect the supernatant;

[0171] Then, the supernatant was filtered with a 0.45 μm filter membrane and loaded onto molecular sieves.

[0172] 2. SuperdexTM30prep grade molecular sieve p...

Embodiment 3

[0181] Embodiment 3 yeast extract detection method

[0182] 1. Determination of bacterial biomass

[0183] Take 50mL of Saccharomyces cerevisiae JCY-P-998 fermentation broth, put it in a 100mL centrifuge tube, centrifuge at 4°C, 8000r / min for 15min, and discard the supernatant;

[0184] Washing with distilled water-centrifugation was repeated 3 times, and the supernatant was discarded; the bacterial cell pellet was dried in a desiccator at 60°C to constant weight, cooled in a desiccator to room temperature and weighed.

[0185] 2. Detection of genetic stability of yeast strain JCY-P-998

[0186] The screened Saccharomyces cerevisiae strain JCY-P-998 was continuously passaged for 8 generations, and the strains of different generations were used for fermentation and culture, and the biomass and polypeptide content of the fermentation products were detected;

[0187] 3. Yeast extract YL-004 polypeptide content determination

[0188] Accurately measure 1mL of the YL-004 solutio...

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Abstract

The invention provides a preparation method and the application of an anti-aging and anti-wrinkle yeast extract. The preparation method comprises the following steps: preparing saccharomyces cerevisiae for producing a yeast extract, and performing fermentation culture to obtain fermentation liquor; wherein the saccharomyces cerevisiae for producing the yeast extract is obtained by screening through any one or at least two of a heparin affinity test, an elastase activity inhibition test, a human fibroblast activity test and a collagen generation test; the fermentation liquor is subjected to molecular sieve purification and heparin affinity chromatography purification, and the anti-aging and anti-wrinkle yeast extract is obtained. According to the preparation method, a high-throughput screening system for breeding of high-heparin affinity yeast is established. Meanwhile, elastinase inhibitory activity, human fibroblast proliferation activity and collagen promotion activity tests are taken as evaluation bases, the yeast extract with the anti-aging and wrinkle-removing effects is obtained, and the yeast extract is taken as a natural and safe additive, and has important economic value.

Description

technical field [0001] The invention relates to the technical field of preparation of daily chemical products, in particular to a preparation method and application of an anti-aging and anti-wrinkle yeast extract. Background technique [0002] In recent years, the anti-aging, firming, anti-wrinkle and repairing cosmetics market has developed very rapidly, and related products have become one of the mainstream products in the beauty and skin care industry; More and more people are looking for biotechnology anti-aging and anti-wrinkle products with high-tech content, natural mildness, safety and effectiveness. [0003] At present, there are many kinds of cosmetic raw materials with clear anti-aging, firming, anti-wrinkle and repairing effects on the market, among which yeast extract and cell growth factor polypeptide raw materials have incomparable advantages over other chemical raw materials. According to data, yeast extract has the advantages of rich nutrition, small molecu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9728A61Q19/08
CPCA61K8/9728A61Q19/08A61K2800/782A61K2800/85
Inventor 于忠国赵振岭刘琳孙大鹏刘广玉陈慧敏
Owner 广州暨创生物医药研究院有限公司
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