Medicine for treating liver cancer and verification model construction method and screening method thereof
A technology for the treatment of liver cancer and screening methods, applied in the field of medicine, can solve the problems of clinical application limitations, side effects, low survival rate, etc., and achieve the effects of enhancing radiotherapy sensitivity, reducing recurrence rate, and improving success rate
Pending Publication Date: 2021-11-12
官键
View PDF0 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Judging from the existing medical clinical situation, chemotherapy (or radiation therapy, radiotherapy) is an important and effective treatment method, but because of its non-specific effect, chemotherapy will kill normal cells indiscriminately. Can produce serious toxic and side effects, and its clinical application has been greatly restricted
[0003] Moreover, in radiotherapy and surgery, radiotherapy resistance is an important reason for liver cancer recurrence or radiotherapy failure, resulting in a low five-year survival rate for patients. Therefore, improving the radiotherapy sensitivity of liver cancer, thereby increasing the success rate of radiotherapy and reducing the recurrence rate has become an urgent problem for medical workers. serious task
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
experiment example 1
[0052] The effect of COMMD10 gene expression on liver cancer cells was tested:
[0053] 1. To construct endogenous stable and low expression COMMD10 HepG2 liver cancer cell line.
[0054] Experimental material: HepG2 liver cancer cell line, purchased from the cell bank of Shanghai Cell Institute, Chinese Academy of Sciences.
[0055] Experimental method and result analysis:
[0056] 1) Cell culture: First, the cells were revived in a 37°C water bath, and then inoculated into cell culture flasks. Cell culture used DMEM medium (FBS, Gibco, Australia) containing 10% fetal bovine serum. Incubator conditions are 37°C, 5% CO 2 .
[0057] 2) Stable strain construction: after recovery of HepG2 cells, wait until the cell density grows to 70-80%, with 2×10 4 Each well was seeded in a 24-well plate and grown overnight in antibiotic-free medium. 10 lentiviruses each with low expression of COMMD10 and control group 8 TU / ml was diluted with 0.5ml ENi.S. solution, added 8mg / ml Polybre...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention relates to a medicine for treating liver cancer. The medicine is characterized in that the medicine can improve the expression level of a COMMD10 gene in cancer cells. The invention further provides a verification model construction method and a screening method of the medicine. The medicine has the advantages of being capable of improving the radiotherapy sensitivity of liver cancer, reducing the recurrence rate, increasing the radiotherapy success rate and the like.
Description
technical field [0001] The invention relates to the field of medicines, in particular to a medicine for treating liver cancer and a verification model construction method and screening method thereof. Background technique [0002] Cancer, also known as tumor, has now become one of the major diseases that endanger human health. In order to better maintain the health of patients, achieving safe and effective anti-tumor therapy has become an important task for medical workers. Judging from the existing medical clinical situation, chemotherapy (or radiation therapy, radiotherapy) is an important and effective treatment method, but because of its non-specific effect, chemotherapy will kill normal cells indiscriminately. Can produce serious toxic and side effects, and its clinical application has been greatly restricted. [0003] Moreover, in radiotherapy surgery, radiotherapy resistance is an important reason for liver cancer recurrence or radiotherapy failure, resulting in a l...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K48/00A61P1/16A61P35/00C12Q1/6886C12Q1/02
CPCA61K45/00A61K48/005A61K48/0008A61P1/16A61P35/00C12Q1/6886G01N33/5011C12Q2600/158G01N2500/10
Inventor 官键杨密吴茜茜
Owner 官键